Difference between revisions of "Part:BBa K1113701:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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We test the targeting sequence of the RuBisCO-GFP (the large and short subunit) in a co-transformation with the Carboxysome biobick (Bba_K1113100)+ RFP, all this in the TOP10 ''E. coli'' strain, then we observe this bacterias with the confocal microscope and look for colocalization of both GFP (targeting sequence) and RFP (carboxysomes).
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how you used this part and how it worked out.
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===Applications of BBa_K1113701===
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[[File:Team_UC_Microscopioconfocal.jpg]]<br>
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The final goal of our constructs it is the delivery of the GFP into the Carboxysome, to observe this we co-transformed TOP10 with both the Carboxysome BioBrick (Bba_K1113100) + RFP (Bba_E1010) and our constructs to observe if they colocalized.
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Figure 1. Confocal Microscopy. Brightfield, GFP filter, RFP filter and merge are shown.
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[[File:Team_UC_Microscopioconfocal.jpg]]
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As observed in Figure 1, two of our constructs display co-localization of green and red fluorescence. Finally we quantify the amount of colocalization of our targeting sequence and obtained (Table 1) that the construct that had the grearest co-localization was C.LG, which is the large subunit of RuBisCO with GFP attached to the carboxil terminal end of the enzime. <br>
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[[File:Nuevaimagen.jpg]]<br>
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Table 1. Quantify of the colocalization.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 22:11, 5 October 2013

We test the targeting sequence of the RuBisCO-GFP (the large and short subunit) in a co-transformation with the Carboxysome biobick (Bba_K1113100)+ RFP, all this in the TOP10 E. coli strain, then we observe this bacterias with the confocal microscope and look for colocalization of both GFP (targeting sequence) and RFP (carboxysomes).

Team UC Microscopioconfocal.jpg
Figure 1. Confocal Microscopy. Brightfield, GFP filter, RFP filter and merge are shown.

As observed in Figure 1, two of our constructs display co-localization of green and red fluorescence. Finally we quantify the amount of colocalization of our targeting sequence and obtained (Table 1) that the construct that had the grearest co-localization was C.LG, which is the large subunit of RuBisCO with GFP attached to the carboxil terminal end of the enzime.
Nuevaimagen.jpg
Table 1. Quantify of the colocalization.

User Reviews

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