Difference between revisions of "Part:BBa K1232000"

Latest revision as of 20:36, 5 October 2013

Green light inducible sfGFP = [PCpcG2-RBS-sfGFP-CcaS](rev)-CcaR

This is the green light responsive GFP output system from the Jeff Tabor plasmid pJT119b.

Usage and Biology

For the biology of this part refer to the [http://2013.igem.org/Team:Washington/LightSensing#Systems Washington 2013 wiki page.]

Native versus Biobrick systems over time.

The first pair of lines in green represent the fluorescence time course of a dual transformation of the native plasmids supplied by the Tabor Lab at Rice University in accordance to the light testing protocol described by the University of Washington iGEM 2013 team on their wiki. There is a measurable difference in GFP expression between the green light and dark conditions.

The second pair of lines in red represent the same experiment but using this biobrick (BBa_K1232000) to express the light sensing proteins and the native plasmid for expression of chromophore expression. There remains a measurable difference in GFP expression between green light and dark conditions.

The third pair of lines in blue represent, again, the same experimental setup but using BBa_K1232004 to express the chromophore producing proteins and the native plasmid for expressing light sensing proteins. Again, there is a measurable difference in GFP expression between green light and dark conditions.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1023
Illegal EcoRI site found at 3898
Illegal XbaI site found at 4599
Illegal SpeI site found at 928
Illegal SpeI site found at 4594
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1023
Illegal EcoRI site found at 3898
Illegal NheI site found at 1652
Illegal SpeI site found at 928
Illegal SpeI site found at 4594
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1023
Illegal EcoRI site found at 3898
Illegal BamHI site found at 4295
Illegal XhoI site found at 6
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1023
Illegal EcoRI site found at 3898
Illegal XbaI site found at 4599
Illegal SpeI site found at 928
Illegal SpeI site found at 4594
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1023
Illegal EcoRI site found at 3898
Illegal XbaI site found at 4599
Illegal SpeI site found at 928
Illegal SpeI site found at 4594
Illegal NgoMIV site found at 2444
Illegal AgeI site found at 988
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 3619

@@ Line 4: / Line 4: @@
 This is the green light responsive GFP output system from the Jeff Tabor plasmid pJT119b.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+For the biology of this part refer to the [http://2013.igem.org/Team:Washington/LightSensing#Systems Washington 2013 wiki page.]
 [[Image:UW_Uwvtabor.png|410px|left|thumb|Native versus Biobrick systems over time.]]
@@ Line 12: / Line 16: @@
-The second pair of lines in red represent the same experiment but using (biobrick name) to express the light sensing proteins and the native plasmid for expression of chromophore expression. There remains a measurable difference in GFP expression between green light and dark conditions.
+The second pair of lines in red represent the same experiment but using this biobrick (BBa_K1232000) to express the light sensing proteins and the native plasmid for expression of chromophore expression. There remains a measurable difference in GFP expression between green light and dark conditions.
-The third pair of lines in blue represent, again, the same experimental setup but using (biobrick name) to express the chromophore producing proteins and the native plasmid for expressing light sensing proteins. Again, there is a measurable difference in GFP expression between green light and dark conditions.
+The third pair of lines in blue represent, again, the same experimental setup but using [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1232004 BBa_K1232004] to express the chromophore producing proteins and the native plasmid for expressing light sensing proteins. Again, there is a measurable difference in GFP expression between green light and dark conditions.