Difference between revisions of "Tutorial:Adding a Basic Part"
 
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Part creation is pretty straightforward if you have some basic information about your part and understand a little  about the Registry's mechanics.
 
Part creation is pretty straightforward if you have some basic information about your part and understand a little  about the Registry's mechanics.
  
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What ''you'' will be doing is uploading a DNA sequence to your Registry [[Help:Sandbox Overview|sandbox]], annotating this sequence, and adding references and other descriptive information.  For example, the DNA sequence might be a coding region for your gene of interest, eg. ''genX'', or a sequence for a promoter, etc. In this tutorial, we take the malE gene sequence which codes the Maltose-binding Protein from e.coli.
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In this tutorial you will be uploading a DNA sequence to your Registry [[Help:Sandbox Overview|sandbox]], annotating this sequence, and adding references and other descriptive information.  For example, the DNA sequence might be a coding region for your gene of interest, eg. ''genX'', or a sequence for a promoter, etc. In this tutorial, we take the malE gene sequence which codes the Maltose-binding Protein from ''E.coli''.
 
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You will need a [https://parts.igem.org/wiki/index.php/Help:Registry_Accounts registry account] if you don't have one.
 
You will need a [https://parts.igem.org/wiki/index.php/Help:Registry_Accounts registry account] if you don't have one.
 
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<big>Once you have an account [[Tutorial:Adding a BioBrick - Step 2|'''click here''']] to start the Tutorial proper</big>
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<big>Once you have an account [[Tutorial:Adding a BioBrick - Step 1|'''click here''']] to start the Tutorial proper</big>
 
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Latest revision as of 22:44, 20 November 2006

Addtutorial.png

Part creation is pretty straightforward if you have some basic information about your part and understand a little about the Registry's mechanics.

In this tutorial you will be uploading a DNA sequence to your Registry sandbox, annotating this sequence, and adding references and other descriptive information. For example, the DNA sequence might be a coding region for your gene of interest, eg. genX, or a sequence for a promoter, etc. In this tutorial, we take the malE gene sequence which codes the Maltose-binding Protein from E.coli.

Before you add your BioBrick to your sandbox, you'll need to collect the following information:

  • A part name
  • The DNA sequence of the part you are making
  • A short description of the part
  • A long description of the part, including references

Remember, this information is ultimately not just for you - while at first you will be using the part's pages to document, keep a record and share information with your group, eventually you'll want to release your part to the Registry. To this end, you should think about who your pages are relevant to initially and adapt them throughout the development process. Eventually, you should look to include the information anyone that wants to use the part will need and want access to. The better you make your parts record, the more likely your part will be used in assemblies.

The Registry will create a parts record - it will check your sequence for unpermissable restriction sites that are incompatible with BioBrick ends. Note: The Registry won't display your sequence with BioBrick ends unless you ask for this, eg. to get the sequence synthesized. It will also calculate some statistics, etc.

The following pages provide a step-by-step guide to adding a basic part to your sandbox:

Step 1: Registry Account

You will need a registry account if you don't have one.

Once you have an account click here to start the Tutorial proper