Difference between revisions of "Part:BBa K1197000"
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<partinfo>BBa_K1197000 short</partinfo> | <partinfo>BBa_K1197000 short</partinfo> | ||
| − | Anti MazF is an antisense RNA producing sequence which is complemantary to MazF (K302033). MazF is a non-specific ribonuclease coding element which kills Bacillus subtilis. Anti MazF element produces an mRNA which is reverse complemantary to mRNA produced by MazF. Thus, Anti Mazf mRNA binds to MazF mRNA and inhibit its activity for coding MazF protein. | + | Anti MazF is an antisense RNA producing sequence which is complemantary to MazF (K302033). MazF is a non-specific ribonuclease coding element which kills ''Bacillus subtilis''. Anti MazF element produces an mRNA which is reverse complemantary to mRNA produced by MazF. Thus, Anti Mazf mRNA binds to MazF mRNA and inhibit its activity for coding MazF protein. |
To show that Anti MazF is functionally works, we performed transformation experiments with kill switch construct (BBa_K1197007) which is IPTG dependent. Since we observed that bacteria reproduced in IPTG including media and they died in media without IPTG. We also saw that when MazF construct (Pveg + RBS SpoVG + MazF + DT) was transformed alone, there was no colony. All this experiments show that Anti MazF can prevent MazF expression in mRNA level which is highly effective. | To show that Anti MazF is functionally works, we performed transformation experiments with kill switch construct (BBa_K1197007) which is IPTG dependent. Since we observed that bacteria reproduced in IPTG including media and they died in media without IPTG. We also saw that when MazF construct (Pveg + RBS SpoVG + MazF + DT) was transformed alone, there was no colony. All this experiments show that Anti MazF can prevent MazF expression in mRNA level which is highly effective. | ||
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'''Gel electrophoresis photo of Anti MazF''' | '''Gel electrophoresis photo of Anti MazF''' | ||
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https://static.igem.org/mediawiki/parts/6/6d/AntiMazF.jpg | https://static.igem.org/mediawiki/parts/6/6d/AntiMazF.jpg | ||
Latest revision as of 04:12, 5 October 2013
Anti MazF - Antisense RNA to inhibit MazF activity in B.subtilis
Anti MazF is an antisense RNA producing sequence which is complemantary to MazF (K302033). MazF is a non-specific ribonuclease coding element which kills Bacillus subtilis. Anti MazF element produces an mRNA which is reverse complemantary to mRNA produced by MazF. Thus, Anti Mazf mRNA binds to MazF mRNA and inhibit its activity for coding MazF protein.
To show that Anti MazF is functionally works, we performed transformation experiments with kill switch construct (BBa_K1197007) which is IPTG dependent. Since we observed that bacteria reproduced in IPTG including media and they died in media without IPTG. We also saw that when MazF construct (Pveg + RBS SpoVG + MazF + DT) was transformed alone, there was no colony. All this experiments show that Anti MazF can prevent MazF expression in mRNA level which is highly effective.
Details for kill switch experiments can be found in own page of kill switch (BBa_K1197007).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Gel electrophoresis photo of Anti MazF
