Difference between revisions of "Part:BBa K1060003:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
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===Characterization of BBa_K1060003===
 
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https://static.igem.org/mediawiki/2013/d/d5/BBa_K1060003.jpg
 
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''E. coli'' BL21(DE3) strain
 
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IPTG: 0,2mM
 
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salicylate: 1mM
 
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chorismate: 1mM
 
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control: BL21(DE3) without plasmid and antbiotics
 
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=== System testing ===
 
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'''Smell Test'''
 
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https://static.igem.org/mediawiki/2013/8/8e/BBa_K1060003_SmellTest_24h_37C.jpg
 
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To test this device several setups for smell tests where made. In the graph shown here the samples were incubated for 24h at 37°C. Methyl salicylate (MS) has a wintergreen odor and can be detected by scent. 11 people smelled each sample independently of one another and answered if they could smell MS or not. For more details on the experimental setup and a discussion of the results go [http://2013.igem.org/Team:KU_Leuven/Project/Glucosemodel/MeS here] under 'smell test'. Chorismate was added to test the salicylate production ([https://parts.igem.org/Part:BBa_J45320 BBa_J45320]) and salicylate to test the wintergreen odor generator ([https://parts.igem.org/Part:BBa_J45120 BBa_J45120]).
 
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===Improvement of <Partinfo>BBa_J45700</partinfo>===
 
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As we recloned this part into standard pSB1C3 backbone, in the sense of ease of use, we <b>improved</b> the brick <Partinfo>BBa_J45700</partinfo>.
 
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 00:20, 29 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQ7040af328d3d79af-partinfo-00000000-QINU UNIQ7040af328d3d79af-partinfo-00000001-QINU

•••••

Sander Wuyts

The KU Leuven iGEM 2013 team tried to perform a qPCR on this part. It was impossible to remove the original plasmid DNA, after RNA isolation, even after several attempts with different DNase treatments. This problem is probably due to the fact that this part was provided in a high copy number backbone. If you want to perform a qPCR yourself, we recommend you to clone this part in another backbone or in the genome itself.

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