Difference between revisions of "Part:BBa K1041004"
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<partinfo>BBa_K1041004 short</partinfo> | <partinfo>BBa_K1041004 short</partinfo> | ||
− | Team NRP-UEA_Norwich 2013 desgined this part using biobrick BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene would be excised from BBa_K1041001 and a gene encoding Gus ligated downstream of the promoter of BBa_K1041001 to create a new biobrick. | + | Team NRP-UEA_Norwich 2013 desgined this part using biobrick BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene would be excised from BBa_K1041001 and a gene encoding Gus ligated downstream of the promoter of BBa_K1041001 to create a new biobrick. The protein product of the GUS gene provides a reporter that can indicate expression of the gene. |
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Latest revision as of 23:11, 4 October 2013
AntG Promoter + Gus gene
Team NRP-UEA_Norwich 2013 desgined this part using biobrick BBa_K1041001. This biobrick contains an Nde1 site after its promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene would be excised from BBa_K1041001 and a gene encoding Gus ligated downstream of the promoter of BBa_K1041001 to create a new biobrick. The protein product of the GUS gene provides a reporter that can indicate expression of the gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 498
Illegal NgoMIV site found at 630
Illegal NgoMIV site found at 1227 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 896