Difference between revisions of "Part:BBa K1150035"
 
(2 intermediate revisions by the same user not shown)
Line 2: Line 2:
 
<partinfo>BBa_K1150035 short</partinfo>
 
<partinfo>BBa_K1150035 short</partinfo>
  
−
..
+
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
−
This device derived from [https://parts.igem.org/Part:BBa_K1150034 BBa_K1150034] can be used in combination with all devices with [https://parts.igem.org/Part:BBa_K1150000 dCas9]. It contains a DNA sequence that transcripts a crRNA that binds complementary to EMX1 target (GGAAGGGCCTGAGTCCGAGCAGAAGAAGAA).
+
! colspan="2" style="background:#FFBF00;"|RNAimer (EMX1 target)
 +
|-
 +
|'''Function'''
 +
|Targeting EMX1 with Cas9
 +
|-
 +
|'''Use in'''
 +
|Mammalians
 +
|-
 +
|'''RFC standard'''
 +
|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
 +
|-
 +
|'''Backbone'''
 +
|pSB1C3<br>
 +
|-
 +
|'''Submitted by'''
 +
|[http://2013.igem.org/Team:Freiburg Freiburg 2013]
 +
|}
 +
 
 +
This device derived from [https://parts.igem.org/Part:BBa_K1150034 BBa_K1150034] can be used in combination with all devices with [https://parts.igem.org/Part:BBa_K1150000 dCas9]. It contains a DNA sequence coding for a crRNA that binds complementary to EMX1 target (GGAAGGGCCTGAGTCCGAGCAGAAGAAGAA).
  
 
For more information about dCas9 devices [http://2013.igem.org/Team:Freiburg klick here].
 
For more information about dCas9 devices [http://2013.igem.org/Team:Freiburg klick here].

Latest revision as of 22:39, 4 October 2013

uniCAS RNAimer to EMX1

RNAimer (EMX1 target)
Function Targeting EMX1 with Cas9
Use in Mammalians
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

This device derived from BBa_K1150034 can be used in combination with all devices with dCas9. It contains a DNA sequence coding for a crRNA that binds complementary to EMX1 target (GGAAGGGCCTGAGTCCGAGCAGAAGAAGAA).

For more information about dCas9 devices [http://2013.igem.org/Team:Freiburg klick here].

Biology and Usage

Freiburg 2013 used this plasmid to test the efficiency of target EMX1 by activating or repressing a SEAP reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216