Difference between revisions of "Part:BBa K1152000"

Latest revision as of 21:07, 4 October 2013

TycA_deltaCom Module from Tyrocidine

This is the CDS for TycA_deltaCom module from the Tyrocidine gene cluster of Brevibacillus parabrevis. It is the first module in gene cluster encoding the non-ribosomal peptide (NRP) synthetase of the cyclic decamer Tyrocidine. This specific module TycA_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP. It consists of an A- (adenylation), a PCP- (peptide-carrier protein) and an E- (epimerization) domain. After tycA follows the tycB gene with three modules.

This biobrick can be used for the design of different NRP synthetases. It was used in the synthetic Tripeptide I of our [http://2013.igem.org/Team:Heidelberg/Project/Tyrocidine project].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2518

@@ Line 5: / Line 5: @@
 It is the first module in gene cluster encoding the non-ribosomal peptide (NRP) synthetase of the cyclic decamer Tyrocidine.
 This specific module TycA_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP.
-It consists of the A- (adenylation), PCP- (peptide-carrier protein) and the E- (epimerization) domain. After tycA follows the tycB gene with three modules.
+It consists of an A- (adenylation), a PCP- (peptide-carrier protein) and an E- (epimerization) domain. After tycA follows the tycB gene with three modules.
 This biobrick can be used for the design of different NRP synthetases.