Difference between revisions of "Part:BBa K1150037"
 
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This device can be used in combination with dCas9. It contains a DNA sequence that transcripts a crRNA that binds complementary to VEGF2 target (GTGTGCAGACGGCAGTCACTAGGGGGCGCT).
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This device derived from [https://parts.igem.org/Part:BBa_K1150034 BBa_K1150034] can be used in combination with all devices with [https://parts.igem.org/Part:BBa_K1150000 dCas9]. It contains a DNA sequence coding for a crRNA that binds complementary to VEGF2 target (GTGTGCAGACGGCAGTCACTAGGGGGCGCT).
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For more information about dCas9 devices [http://2013.igem.org/Team:Freiburg klick here].
  
 
==Biology and Usage==
 
==Biology and Usage==
  
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Freiburg 2013 used this plasmid to test the efficiency of target VEGF2. Therefore this target was also cloned in front of a promoter, that on its part is located in front of a SEAP reporter gene.
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Freiburg 2013 used this plasmid to test the efficiency of target VEGF2 by activating or repressing a SEAP reporter gene.
  
 
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Latest revision as of 22:34, 4 October 2013

uniCAS RNaimer to target VEGF2


RNAimer (VEGF2 target)
Function Targeting VEGF2 with Cas9
Use in Mammalians
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]

This device derived from BBa_K1150034 can be used in combination with all devices with dCas9. It contains a DNA sequence coding for a crRNA that binds complementary to VEGF2 target (GTGTGCAGACGGCAGTCACTAGGGGGCGCT).

For more information about dCas9 devices [http://2013.igem.org/Team:Freiburg klick here].

Biology and Usage

Freiburg 2013 used this plasmid to test the efficiency of target VEGF2 by activating or repressing a SEAP reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216

Literature