Difference between revisions of "Part:BBa K1150032"

Latest revision as of 23:14, 4 October 2013

uniCAS UV Light Switch Part II - Histone Modifier

CMV:COP1-Linker-G9a-NLS:BGH
Function	Histone modification domain of UVB light induced gene expression control
Use in	Mammalians
RFC standard	RFC 25
Backbone	pSB1C3
Submitted by	[http://2013.igem.org/Team:Freiburg Freiburg 2013]

Introduction

This part contains the protein COP1 fused to the Methyl-Tranferase G9a. It's an interaction partner of dCas9-UVR8 and part of the UVB light controlled mechanism to repress gene expression. You will be able to repress any desired gene by a short UV-light pulse.

For more information and experimental data have a look at our [http://2013.igem.org/Team:Freiburg/Project/induction#light light] and [http://2013.igem.org/Team:Freiburg/Project/effector#epigenetics epigenetic] project page.

Biology & Usage

COP1 is a protein that was first described in the model organism Arabidopsis thaliana. It will bind to the UV-light receptor UVR8 after illumination [1]. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation. For this UVB light controled gene repression system you need a combination of these BioBricks: dCas9-UVR8, COP1-G9a and RNaimer.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 576
Illegal BglII site found at 2231
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1373
Illegal BsaI.rc site found at 1450
Illegal SapI.rc site found at 962

References

[1] Müller, K.; Engesser, R.; Schulz, S.; Steinberg, T.; Tomakidi, P.; Weber, C. C. et al. (Nucleid Acids Research 2013): Multi-chromatic control of mammalian gene expression and signaling

@@ Line 3: / Line 3: @@
 {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
-! colspan="2" style="background:#FFBF00;"|CMV:COP1-G9a:BGH
+! colspan="2" style="background:#FFBF00;"|CMV:COP1-Linker-G9a-NLS:BGH
 |-
 |'''Function'''
@@ Line 27: / Line 27: @@
 ==Introduction==
-This part contains the protein COP1 fused to the Methyl-Tranferase G9a. It's an interaction partner of [https://parts.igem.org/Part:BBa_K1150029 dCas9-UVR8] and  part of the UVB light controlled mechanism to repress gene expression. You will be able to repress any desired gene by a short UV-light pulse.
+This part contains the protein [https://parts.igem.org/Part:BBa_K1150007 COP1] fused to the Methyl-Tranferase [https://parts.igem.org/Part:BBa_K1150003 G9a]. It's an interaction partner of [https://parts.igem.org/Part:BBa_K1150029 dCas9-UVR8] and  part of the UVB light controlled mechanism to repress gene expression. You will be able to repress any desired gene by a short UV-light pulse.
+For more information and experimental data have a look at our [http://2013.igem.org/Team:Freiburg/Project/induction#light light] and [http://2013.igem.org/Team:Freiburg/Project/effector#epigenetics epigenetic] project page.
 ==Biology & Usage==
-COP1 is a protein that was first described in the model organism ''Arabidopsis thaliana''. It will bind to the UV-light receptor UVR8 after illumination [1]. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation. For this UVB light controled gene repression system you need a combination of these BioBricks: [https://parts.igem.org/Part:BBa_K1150029 Cas9-UVR8], [https://parts.igem.org/Part:BBa_K1150032 COP1-G9a] and [https://parts.igem.org/Part:BBa_K1150034 RNaimer].
+COP1 is a protein that was first described in the model organism ''Arabidopsis thaliana''. It will bind to the UV-light receptor UVR8 after illumination [1]. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation. For this UVB light controled gene repression system you need a combination of these BioBricks: [https://parts.igem.org/Part:BBa_K1150029 dCas9-UVR8], [https://parts.igem.org/Part:BBa_K1150032 COP1-G9a] and [https://parts.igem.org/Part:BBa_K1150034 RNaimer].