Difference between revisions of "Part:BBa K1150031"

Latest revision as of 23:13, 4 October 2013

uniCAS UV Light Switch Part II - Repressor

CMV:COP1-Linker-KRAB-NLS:BGH
Function	Repression domain of UVB light induced gene expression control
Use in	Mammalians
RFC standard	RFC 25
Backbone	pSB1C3
Submitted by	[http://2013.igem.org/Team:Freiburg Freiburg 2013]

The fusion protein COP1-KRAB is an interaction partner of dCas9-UVR8 and part of the UVB light controlled mechanism to repress gene expression. First the dCas9-UVR8 fusion protein binds specifically to a DNA target sequence. If the system is exposed to UVB light (311 nm), the UVR8 receptor of UV changes its configuration and recruits this COP1-KRAB protein. This represses gene expression controlled by UVB light.

For more information and experimental data have a look at our [http://2013.igem.org/Team:Freiburg/Project/induction#light light] and [http://2013.igem.org/Team:Freiburg/Project/effector#repression repression] project page.

Biology and Usage

E3-ubiquitin ligase Constitutively Photomorphogenic 1 (COP1) is the interaction partner of ultraviolet B (UVB) light receptor UVR8 [1]. When UVB light is perceived by the UVR8 photoreceptor it monomerizes and therefore changes in its open confuguration. Not till then it recruits COP1. For this UVB light controled gene activation system you need a combination of these BioBricks: dCas9-UVR8, COP1-KRAB and RNaimer.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 576
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1373
Illegal BsaI.rc site found at 1450
Illegal BsaI.rc site found at 1876
Illegal SapI.rc site found at 962
Illegal SapI.rc site found at 1840

References

[1] Rizzini L., Favory J.-J., Cloix C., Faggionato D., O'Hara A., Kaiserli E., Baumeister R., Schäfer E., Nagy F., Jenkins G. I., Ulm R. (2011). Perception of UV-B by the Arabidopsis UVR8 protein. Science 332

@@ Line 3: / Line 3: @@
 {| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
-! colspan="2" style="background:#FFBF00;"|CMV:COP1-KRAB:BGH
+! colspan="2" style="background:#FFBF00;"|CMV:COP1-Linker-KRAB-NLS:BGH
 |-
 |'''Function'''
@@ Line 22: / Line 22: @@
 |}
-The fusion protein COP1-KRAB is an interaction partner of [https://parts.igem.org/Part:BBa_K1150029 dCas9-UVR8] and  part of the UVB light controlled mechanism to repress gene expression. First the dCas9-UVR8 fusion protein binds specifically to a DNA target sequence. If the system is exposed to UVB light (311 nm), the UVR8 receptor of UV changes its configuration and recruits this COP1-KRAB protein. This represses gene expression controlled by UVB light. Have a look at our [http://2013.igem.org/Team:Freiburg/Project/induction#light light project page].
+The fusion protein COP1-KRAB is an interaction partner of [https://parts.igem.org/Part:BBa_K1150029 dCas9-UVR8] and  part of the UVB light controlled mechanism to repress gene expression. First the dCas9-UVR8 fusion protein binds specifically to a DNA target sequence. If the system is exposed to UVB light (311 nm), the UVR8 receptor of UV changes its configuration and recruits this COP1-KRAB protein. This represses gene expression controlled by UVB light.
+For more information and experimental data have a look at our [http://2013.igem.org/Team:Freiburg/Project/induction#light light] and [http://2013.igem.org/Team:Freiburg/Project/effector#repression repression] project page.
 ==Biology and Usage==
-E3-ubiquitin ligase Constitutively Photomorphogenic 1 (COP1) is the interaction partner of ultraviolet B (UVB) light receptor [https://parts.igem.org/Part:BBa_K1150006 UVR8] [1]. When UVB light is perceived by the UVR8 photoreceptor it monomerizes and therefore changes in its open confuguration. Not till then it recruits COP1.
+E3-ubiquitin ligase Constitutively Photomorphogenic 1 ([https://parts.igem.org/Part:BBa_K1150007 COP1]) is the interaction partner of ultraviolet B (UVB) light receptor [https://parts.igem.org/Part:BBa_K1150006 UVR8] [1]. When UVB light is perceived by the UVR8 photoreceptor it monomerizes and therefore changes in its open confuguration. Not till then it recruits COP1.
-For this UVB light controled gene activation system you need a combination of these BioBricks: [https://parts.igem.org/Part:BBa_K1150029 Cas9-UVR8], [https://parts.igem.org/Part:BBa_K1150031 COP1-KRAB] and [https://parts.igem.org/Part:BBa_K1150034 RNaimer].
+For this UVB light controled gene activation system you need a combination of these BioBricks: [https://parts.igem.org/Part:BBa_K1150029 dCas9-UVR8], [https://parts.igem.org/Part:BBa_K1150031 COP1-KRAB] and [https://parts.igem.org/Part:BBa_K1150034 RNaimer].
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 ==Sequence and Features==
 </span>