Difference between revisions of "Part:BBa K1150032"
 
(5 intermediate revisions by 2 users not shown)
Line 3: Line 3:
  
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
 
{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
−
! colspan="2" style="background:#FFBF00;"|CMV:COP1-G9a:BGH
+
! colspan="2" style="background:#FFBF00;"|CMV:COP1-Linker-G9a-NLS:BGH
 
|-
 
|-
 
|'''Function'''
 
|'''Function'''
Line 27: Line 27:
  
 
==Introduction==
 
==Introduction==
−
This part contains the protein COP1 fused to the Methyl-Tranferase G9a. It's an interaction partner of [https://parts.igem.org/Part:BBa_K1150029 dCas9-UVR8] and  part of the UVB light controlled mechanism to repress gene expression. You will be able to repress any desired gene by a short UV-light pulse.
+
This part contains the protein [https://parts.igem.org/Part:BBa_K1150007 COP1] fused to the Methyl-Tranferase [https://parts.igem.org/Part:BBa_K1150003 G9a]. It's an interaction partner of [https://parts.igem.org/Part:BBa_K1150029 dCas9-UVR8] and  part of the UVB light controlled mechanism to repress gene expression. You will be able to repress any desired gene by a short UV-light pulse.
 +
 
 +
For more information and experimental data have a look at our [http://2013.igem.org/Team:Freiburg/Project/induction#light light] and [http://2013.igem.org/Team:Freiburg/Project/effector#epigenetics epigenetic] project page.
  
 
==Biology & Usage==
 
==Biology & Usage==
−
COP1 is a protein that was first described in the model organism ''Arabidopsis thaliana''. It will bind to the UV-light receptor UVR8 after illumination. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation. For this UVB light controled gene repression system you need a combination of these BioBricks: [https://parts.igem.org/Part:BBa_K1150029 Cas9-UVR8], [https://parts.igem.org/Part:BBa_K1150032 COP1-G9a] and [https://parts.igem.org/Part:BBa_K1150034 RNaimer].
+
COP1 is a protein that was first described in the model organism ''Arabidopsis thaliana''. It will bind to the UV-light receptor UVR8 after illumination [1]. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation. For this UVB light controled gene repression system you need a combination of these BioBricks: [https://parts.igem.org/Part:BBa_K1150029 dCas9-UVR8], [https://parts.igem.org/Part:BBa_K1150032 COP1-G9a] and [https://parts.igem.org/Part:BBa_K1150034 RNaimer].
  
  
Line 39: Line 41:
  
 
== References ==
 
== References ==
−
Muller, K.; Engesser, R.; Schulz, S.; Steinberg, T.; Tomakidi, P.; Weber, C. C. et al. (Nucleid Acids Research 2013): Multi-chromatic control of mammalian gene expression and signaling
+
[1] Müller, K.; Engesser, R.; Schulz, S.; Steinberg, T.; Tomakidi, P.; Weber, C. C. et al. (Nucleid Acids Research 2013): Multi-chromatic control of mammalian gene expression and signaling
  
  

Latest revision as of 23:14, 4 October 2013

uniCAS UV Light Switch Part II - Histone Modifier

CMV:COP1-Linker-G9a-NLS:BGH
Function Histone modification domain

of UVB light induced gene

expression control

Use in Mammalians
RFC standard RFC 25
Backbone pSB1C3
Submitted by [http://2013.igem.org/Team:Freiburg Freiburg 2013]


Introduction

This part contains the protein COP1 fused to the Methyl-Tranferase G9a. It's an interaction partner of dCas9-UVR8 and part of the UVB light controlled mechanism to repress gene expression. You will be able to repress any desired gene by a short UV-light pulse.

For more information and experimental data have a look at our [http://2013.igem.org/Team:Freiburg/Project/induction#light light] and [http://2013.igem.org/Team:Freiburg/Project/effector#epigenetics epigenetic] project page.

Biology & Usage

COP1 is a protein that was first described in the model organism Arabidopsis thaliana. It will bind to the UV-light receptor UVR8 after illumination [1]. Thereby recruiting G9a to its target locus. Subsequently the local DNA-expression will be repressed by DNA-methylation. For this UVB light controled gene repression system you need a combination of these BioBricks: dCas9-UVR8, COP1-G9a and RNaimer.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 2231
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1373
    Illegal BsaI.rc site found at 1450
    Illegal SapI.rc site found at 962

References

[1] Müller, K.; Engesser, R.; Schulz, S.; Steinberg, T.; Tomakidi, P.; Weber, C. C. et al. (Nucleid Acids Research 2013): Multi-chromatic control of mammalian gene expression and signaling