Difference between revisions of "Part:BBa K1075013:Design"
 
Line 9: Line 9:
  
 
===Source===
 
===Source===
−
It was amplified from a plasmid from Addgene - provided by Klaus Hahn. LOV2 originally comes from Avena sativa while the ipaA peptide comes from Shigella spp.  
+
It was amplified from a plasmid from Addgene - provided by Klaus Hahn. LOV2 originally comes from ''Avena sativa'' while the ipaA peptide comes from ''Shigella spp.''.  
  
 
===References===
 
===References===
 
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334866/]Lungu et al. (2012) Designing photoswitchable peptides using the AsLOV2 domain
 
[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334866/]Lungu et al. (2012) Designing photoswitchable peptides using the AsLOV2 domain

Latest revision as of 10:47, 4 October 2013

AsLOV2-ipaA(L623A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This fusion protein was designed by Lungu et al. [1]

Source

It was amplified from a plasmid from Addgene - provided by Klaus Hahn. LOV2 originally comes from Avena sativa while the ipaA peptide comes from Shigella spp..

References

[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334866/]Lungu et al. (2012) Designing photoswitchable peptides using the AsLOV2 domain