Difference between revisions of "Part:BBa K1060003:Experience"

(Applications of BBa_K1060003)
(System testing)
 
(16 intermediate revisions by 3 users not shown)
Line 2: Line 2:
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
how you used this part and how it worked out.
 
how you used this part and how it worked out.
 
===Characterization of BBa_K1060003===
 
 
https://static.igem.org/mediawiki/2013/d/d5/BBa_K1060003.jpg
 
 
''E. coli'' BL21(DE3) strain
 
 
IPTG: 0,2mM
 
 
salicylate: 1mM
 
 
chorismate: 1mM
 
 
control: BL21(DE3) without plasmid and antbiotics
 
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 00:20, 29 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

User Reviews

UNIQbd2f3575f924320c-partinfo-00000000-QINU UNIQbd2f3575f924320c-partinfo-00000001-QINU

•••••

Sander Wuyts

The KU Leuven iGEM 2013 team tried to perform a qPCR on this part. It was impossible to remove the original plasmid DNA, after RNA isolation, even after several attempts with different DNase treatments. This problem is probably due to the fact that this part was provided in a high copy number backbone. If you want to perform a qPCR yourself, we recommend you to clone this part in another backbone or in the genome itself.

;