Difference between revisions of "Part:BBa K1033225"

Latest revision as of 20:51, 4 October 2013

Promoter CP44

CP44 is the strongest promoter in the CP collection with the strength of 4.05 compared to the promoter J23101 in standard parts (J23101 was used as reference and its strength were put to 1).

Above you can see a diagram of all the CP promoters strength relative to J23101 [RPU]. The CP promoter parts is a collection of constitutive promoters characterized in both E. coli and Lactococcus lactis by Jensen and Hammer (1998). In E. coli it has also been characterized by us in a fluorescence-activated cell sorting machine (FACS) with BFP using the constitutive promoter J23101 in the standard parts as a reference.

These promoters have further been used successfully in several parts in our project, including BBa_K1033000 (TAL), BBa_K1033002 (STS) and BBa_K1033281 (amilCP).

In the article by Jensen and Hammer they stated that these promoters were based on the consensus sequence of promoters in gram-positive bacterium L. lactis, though there are no obvious reasons why they shouldn’t work in other organisms. Jensen and Hammer characterized them in both L. lactis and gram-negative bacterium E. coli using the reporter gene lacLM. This indicates that there is a possibility that these promoters are near universally applicable to prokaryotic organisms in general.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1033225 short</partinfo>
-CP44 is the strongest promoter in the CP collection with the strength of 4.0532 compared to the promoter J23101 in standard parts (J23101 was used as reference and its strength were put to 1).
+CP44 is the strongest promoter in the CP collection with the strength of 4.05 compared to the promoter J23101 in standard parts (J23101 was used as reference and its strength were put to 1).
 https://static.igem.org/mediawiki/2013/6/64/Uppsala2013_Promoterdiagram.png
-Above you can see a diagram of all the CP promoters strength relative to J23101 [RPU]. The CP promoter parts is a collection of constitutive promoters characterized in both ''E. coli'' and ''Lactococcus lactis'' by Jensen and Hammer (1998). In ''E. coli'' it has also been characterized in a fluorescence-activated cell sorting machine (FACS) with BFP using the constitutive promoter J23101 in the standard parts as a reference.
+Above you can see a diagram of all the CP promoters strength relative to J23101 [RPU]. The CP promoter parts is a collection of constitutive promoters characterized in both ''E. coli'' and ''Lactococcus lactis'' by Jensen and Hammer (1998). In ''E. coli'' it has also been characterized by us in a fluorescence-activated cell sorting machine (FACS) with BFP using the constitutive promoter J23101 in the standard parts as a reference.
+These promoters have further been used successfully in several parts in our project, including [https://parts.igem.org/Part:BBa_K103300 BBa_K1033000] (TAL), [https://parts.igem.org/Part:BBa_K1033002 BBa_K1033002] (STS) and [https://parts.igem.org/Part:BBa_K1033281 BBa_K1033281] (amilCP).
 In the article by Jensen and Hammer they stated that these promoters were based on the consensus sequence of promoters in gram-positive bacterium ''L. lactis'', though there are no obvious reasons why they shouldn’t work in other organisms. Jensen and Hammer characterized them in both ''L. lactis'' and gram-negative bacterium ''E. coli'' using the reporter gene lacLM. This indicates that there is a possibility that these promoters are near universally applicable to prokaryotic organisms in general.