Difference between revisions of "Part:BBa K1075028"
 
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<partinfo> pJD427-plac2-RBS34-mcherry-TT short</partinfo>
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<partinfo>BBa_K1075028 short</partinfo>
  
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The part contains the promoter pLac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a ecSspB split system.
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<!-- Add more about the biology of this part here-->
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===construction===
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The part contains the promoter plac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a SspB split system.
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===biology===
 
===biology===
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The plasmid pJD427 contains the two parts of splitted SspB, which can be fused (and thus regain function) by addition of rapamycin. [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220803/]]
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The plasmid pJD427 contains the two parts of split ecSspB, which can be fused (and thus regain function) by addition of rapamycin. [[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220803/]]
  
  
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===application===
 
===application===
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This plasmid is a negative control to the pJD427-pLac2-RBS34-mCherry-ssrA-TT plasmid. [[https://parts.igem.org/Part:BBa_K1075027]]
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This plasmid is a negative control to the pJD427-pLac2-RBS34-mCherry-ecssrA(DAS+4)-TT plasmid. [[https://parts.igem.org/Part:BBa_K1075027]]
  
 
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Latest revision as of 01:33, 5 October 2013

SplitSspB(Rapamycin inducable)-pLac-RBS34-mCherry-TT

The part contains the promoter pLac, a ribosomal binding site, the red fluorescent protein mCherry and a double terminator. It is integrated in the plasmid pJD427, which contains a ecSspB split system.


biology

The plasmid pJD427 contains the two parts of split ecSspB, which can be fused (and thus regain function) by addition of rapamycin. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3220803/


plac is a IPTG inducible promoter.


The ribosomal binding side is a sequence of mRNA where the ribosome binds to start translation.


mCherry is a red fluorescent protein with the excitation maximum at 587 nm and the emission maximum at 610 nm. http://www.ncbi.nlm.nih.gov/pubmed/15558047


The double terminator stops the transcription at this point.


application

This plasmid is a negative control to the pJD427-pLac2-RBS34-mCherry-ecssrA(DAS+4)-TT plasmid. [[1]]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 102
    Illegal BsaI.rc site found at 743