Difference between revisions of "Part:BBa K1223014:Design"

 
(→‎Design Notes)
 
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this is a part of the pyrrolysine incorporation machinery.
 
this is a part of the pyrrolysine incorporation machinery.
  
 +
this tRNA is charged by pyrrolysyl-tRNA synthetase (pylS gene) to incorporate the amino acid pyro lysine.
 +
the tRNA has CUA anticodon that recognizes the TAG (UAG) codon on the mRNA during the translation process.
  
 +
pylS CDS can be found in part BBa_K1223013.
  
 
===Source===
 
===Source===
  
−
Methanosarcina barkeri str. Fusaro genomic sequence.
+
derived from the genomic sequence of Methanosarcina barkeri. gene name - pylT
 +
 
 +
clasification:
 +
Archaea : Euryarchaeota : Methanomicrobia : Methanosarcinales : Methanosarcinaceae : Methanosarcina
  
 
===References===
 
===References===
 +
 +
Srinivasan,G., James,C.M. and Krzycki,J.A., Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA, Science 296 (5572), 1459-1462 (2002)
 +
Milles, S.; Tyagi, S.; Banterle, N.; Koehler, C.; VanDelinder, V.; Plass, T.; Neal, A. P.; Lemke, E. A., Journal of the American Chemical Society 2012, 134 (11), 5187-95.

Latest revision as of 16:13, 3 October 2013


tRNA-Pyl (pylT) gene from Methanosarcina barkeri str. Fusaro


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

this is a part of the pyrrolysine incorporation machinery.

this tRNA is charged by pyrrolysyl-tRNA synthetase (pylS gene) to incorporate the amino acid pyro lysine. the tRNA has CUA anticodon that recognizes the TAG (UAG) codon on the mRNA during the translation process.

pylS CDS can be found in part BBa_K1223013.

Source

derived from the genomic sequence of Methanosarcina barkeri. gene name - pylT

clasification: Archaea : Euryarchaeota : Methanomicrobia : Methanosarcinales : Methanosarcinaceae : Methanosarcina

References

Srinivasan,G., James,C.M. and Krzycki,J.A., Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA, Science 296 (5572), 1459-1462 (2002) Milles, S.; Tyagi, S.; Banterle, N.; Koehler, C.; VanDelinder, V.; Plass, T.; Neal, A. P.; Lemke, E. A., Journal of the American Chemical Society 2012, 134 (11), 5187-95.