Difference between revisions of "Part:BBa K1065101"

Latest revision as of 18:48, 3 October 2013

AraCpBAD + BSMT1

The part BBa_K1065100 is under the control of the inducible promoter AraC-pBAD. This promoter allows to produce methyl salicylate starting from salicylic acid when Arabinose is added to the cell culture.

This part was cloned by the iGEM UniTN Trento 2013 team for the construction of Wintergreen Devices BBa_K1065102 and BBa_K1065106.

Usage and Biology

BSMT1 catalyzes the conversion of salicylic acid to methyl salicylate, which has a wintergreen smell.

The arabinose inducible promoter BBa_K731201 was fully characterized by iGEM UniTN Trento 2012.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
Illegal BamHI site found at 1893
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1434
Illegal SapI site found at 961

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1065101 short</partinfo>
-The part <partinfo>BBa_K1065100</partinfo> is under the control of the inducible promoter AraC-pBAD. This promoter allows to produce methyl salicylate starting from salicylic acid when 5 mM of Arabinose is added
-to the culture.
+The part <partinfo>BBa_K1065100</partinfo> is under the control of the inducible promoter AraC-pBAD. This promoter allows to produce methyl salicylate starting from salicylic acid when Arabinose is added to the cell culture.
 This part was cloned by the iGEM UniTN Trento 2013 team for the construction of
-Wintergreen Device <partinfo>BBa_K1065106</partinfo>.
+Wintergreen Devices <partinfo>BBa_K1065102</partinfo> and <partinfo>BBa_K1065106</partinfo>.
@@ Line 17: / Line 18: @@
 </html>
-The arabinose inducible promoter <partinfo>BBa_K731201</partinfo> was fully characterized by iGEM UniTN Trento 2012.
 <!-- Uncomment this to enable Functional Parameter display