Difference between revisions of "Part:BBa K1033260"
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| − | This is a toxin-antitoxin system (pem) together with a natural putative promotor from the Lactobacillus plantarum NC7 plasmid p256. The main use of the toxin-antitoxin system is to make the plasmid more stabile without having to rely on antibiotic resistance. The antitoxin has a shorter halflife then the antitoxin. If the cell were to lose the plasmid the toxin will remain longer in the cell and thereby kill it. It has been shown to increase segregational stability under non-selective pressure. The system has experimentally been shown to allow 88-100% retention of a plasmid after 80 generations. | + | This is a toxin-antitoxin system (pem) together with a natural putative promotor from the Lactobacillus plantarum NC7 plasmid p256. The main use of the toxin-antitoxin system is to make the plasmid more stabile without having to rely on antibiotic resistance. The antitoxin has a shorter halflife then the antitoxin. If the cell were to lose the plasmid the toxin will remain longer in the cell and thereby kill it. It has been shown to increase segregational stability under non-selective pressure. The system has experimentally been shown to allow 88-100% retention of a plasmid after 80 generations.<sup> [1] </sup> |
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| + | [1] Plasmid p256 from Lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin–antitoxin-like plasmid maintenance system, Microbiology, Elisabeth Sorvig et al. September 30 2004 | ||
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Latest revision as of 19:57, 3 October 2013
Toxin-antitoxin system from Lactobacillus plantarum
This is a toxin-antitoxin system (pem) together with a natural putative promotor from the Lactobacillus plantarum NC7 plasmid p256. The main use of the toxin-antitoxin system is to make the plasmid more stabile without having to rely on antibiotic resistance. The antitoxin has a shorter halflife then the antitoxin. If the cell were to lose the plasmid the toxin will remain longer in the cell and thereby kill it. It has been shown to increase segregational stability under non-selective pressure. The system has experimentally been shown to allow 88-100% retention of a plasmid after 80 generations. [1]
[1] Plasmid p256 from Lactobacillus plantarum represents a new type of replicon in lactic acid bacteria, and contains a toxin–antitoxin-like plasmid maintenance system, Microbiology, Elisabeth Sorvig et al. September 30 2004
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 158
Illegal SpeI site found at 534 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 158
Illegal SpeI site found at 534 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 158
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 158
Illegal SpeI site found at 534 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 158
Illegal SpeI site found at 534 - 1000COMPATIBLE WITH RFC[1000]