Difference between revisions of "Part:BBa K1122000"
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| − | SinR protein is the master regulator of biofilm formation in Bacillus subtillis. In the active state, it represses biofilms. | + | SinR protein is the master regulator of biofilm formation in Bacillus subtillis. In the active state, it represses biofilms. The regulatory pathway in which SinR lies is displayed in Figure 1. SinI inhibits the action of SinR by disrupting its' ability to bind DNA. The downstream regulatory network contains the exopolysaccharide (<i>eps</i>) and <i>yqxM</i> (containing an EPS bridging protein TasA) operons. |
| + | https://static.igem.org/mediawiki/2013/3/35/F1.medium.gif | ||
| + | '' '''Figure 1''' '' The regulatory pathway of biofilm formation in <i>Bacilus subtilis</i> (From Winkelman et al. 2009) | ||
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| + | Winkelman J. T., Blair K. M., Kearns D. B. (2009) RemA (YlzA) and RemB (YaaB) Regulate Extracellular Matrix Operon Expression and Biofilm Formation in Bacillus subtilis<i>, J. Bacteriol.</i>, vol 191 no. 12, June, pp. 3981-3991. | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | The gene is 333bp long and can be seen clearly on a gel when cloned from <i>B. subtilis</i>: | |
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| + | https://static.igem.org/mediawiki/parts/5/59/AggregationResults2.png | ||
Latest revision as of 20:44, 4 October 2013
SinR transcription factor
SinR protein is the master regulator of biofilm formation in Bacillus subtillis. In the active state, it represses biofilms. The regulatory pathway in which SinR lies is displayed in Figure 1. SinI inhibits the action of SinR by disrupting its' ability to bind DNA. The downstream regulatory network contains the exopolysaccharide (eps) and yqxM (containing an EPS bridging protein TasA) operons.
Figure 1 The regulatory pathway of biofilm formation in Bacilus subtilis (From Winkelman et al. 2009)
Winkelman J. T., Blair K. M., Kearns D. B. (2009) RemA (YlzA) and RemB (YaaB) Regulate Extracellular Matrix Operon Expression and Biofilm Formation in Bacillus subtilis, J. Bacteriol., vol 191 no. 12, June, pp. 3981-3991.
Usage and Biology
The gene is 333bp long and can be seen clearly on a gel when cloned from B. subtilis:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]