Difference between revisions of "Part:BBa K1051000"
 
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https://static.igem.org/mediawiki/2013/0/0d/Reporter.jpg
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===Modified===
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https://static.igem.org/mediawiki/2013/thumb/e/e9/Remove_codon.jpg/800px-Remove_codon.jpg
 
Fluorescence proteins have been modified to RFC[23], and stop codons have been removed for fusion protein purpose.
 
Fluorescence proteins have been modified to RFC[23], and stop codons have been removed for fusion protein purpose.
 +
 +
===Result===
 +
https://static.igem.org/mediawiki/2013/0/0d/Reporter.jpg

Latest revision as of 04:14, 3 October 2013

Stop codon free RFP in RFC[23] standard

Red fluorescent protein with assembly standard 23 is engineered mutant of red fluorescent protein from Discosoma striata (coral).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Modified

800px-Remove_codon.jpg Fluorescence proteins have been modified to RFC[23], and stop codons have been removed for fusion protein purpose.

Result

Reporter.jpg