Difference between revisions of "Part:BBa K1104202:Experience"
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This part HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104201 Part:BBa_K1104201]) was used as sensor to ROS(Reactive Oxygen Species). More details about ROS-induced promoters are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].
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HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104202 Part:BBa_K1104202]) is an OxyR-induced promoter, and was used as sensor to ROS(Reactive Oxygen Species).
  
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HemH promoter +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242]) includes promoter HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K110424 Part:BBa_K1104242]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 BBa_E0840].
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[[File: NYMU_42.png|frame|center|'''HemH promoter +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242])''']]
  
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[[File: NYMU_42.png|frame|center|'''HemH promoter +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104241 Part:BBa_K1104242])''']]
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We designed the device: HemH promoter +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242]) includes HemH promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induced HemH promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identified the strength.
  
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After HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104201 Part:BBa_K1104201]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
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In this device, after HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104202 Part:BBa_K1104202]) is activated, GFP generator [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840] takes as input a transcriptional signal (PoPS) and produce as output GFP (green fluorescent protein). We induced HemH promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and measured the expression of GFP, and identified the strength.  
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==Results==
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This is the relative promoter strength of BBa_K1104202 under different H<sub>2</sub>0<sub>2</sub> concentrations tested using BBa_K11042042 (BBa_K1104202 +E0840) and found no significant trend where the higher the H<sub>2</sub>0<sub>2</sub> concentration, the higher the promoter expression:
  
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[[File: hem.png|frame|center|(Error bars represent the 95% confidence interval)]]
  
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==Results==
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Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:
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[[File: NYMU_all1.png|frame|center|Comparison between ahpC and its derived promoters.(Error bars represent the 95% confidence interval)]]
  
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More details about ROS-induced promoters are in page: [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae''].
  
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==Discussion==
 
  
 
===Applications of BBa_K1104202===
 
===Applications of BBa_K1104202===

Latest revision as of 21:44, 27 October 2013


HemHp (Part:BBa_K1104202) is an OxyR-induced promoter, and was used as sensor to ROS(Reactive Oxygen Species).

HemH promoter +E0840 (Part:BBa_K1104242)

We designed the device: HemH promoter +E0840 (Part:BBa_K1104242) includes HemH promoter (Part:BBa_K1104242) and Part:BBa_E0840, induced HemH promoter by different concentrations of H2O2, and identified the strength.

In this device, after HemHp (Part:BBa_K1104202) is activated, GFP generator Part:BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output GFP (green fluorescent protein). We induced HemH promoter by different concentrations of H2O2, and measured the expression of GFP, and identified the strength.

Results

This is the relative promoter strength of BBa_K1104202 under different H202 concentrations tested using BBa_K11042042 (BBa_K1104202 +E0840) and found no significant trend where the higher the H202 concentration, the higher the promoter expression:

(Error bars represent the 95% confidence interval)

Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:

Comparison between ahpC and its derived promoters.(Error bars represent the 95% confidence interval)

More details about ROS-induced promoters are in page: [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].


Applications of BBa_K1104202

User Reviews

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