Difference between revisions of "Part:BBa K1104206:Experience"
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| − | We tested the improved promoter: AhpCpD1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. | + | This part: AhpCpD1 is one of the improvement versions of BioBrick Part: ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCpD1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104206 Part:BBa_K1104206]) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae'']. |
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| + | [[File: NYMU_46.png|frame|center|'''AhpCpD1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104246 Part:BBa_K1104246])''']] | ||
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| + | We use the device: AhpCpD1 +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104246 Part:BBa_K1104246]) includes promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104206 Part:BBa_K1104206]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induce the promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identify the strength. After AhpCpD1 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104206 Part:BBa_K1104206]) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP. | ||
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| + | We tested the improved promoter: AhpCpD1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing. | ||
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==Results== | ==Results== | ||
| − | + | This is the relative promoter strength of BBa_K1104206 under different H<sub>2</sub>0<sub>2</sub> concentrations tested using BBa_K11042046 (BBa_K1104206 +E0840) and found that there is a trend where the higher the H<sub>2</sub>0<sub>2</sub> concentration, the higher the promoter expression: | |
[[File: NYMU_AhpCpD1_strength.png|frame|center]] | [[File: NYMU_AhpCpD1_strength.png|frame|center]] | ||
| − | [[File: NYMU_all1.png|frame|center]] | + | Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101: |
| + | [[File: NYMU_all1.png|frame|center|Comparison between ahpC and its improvements. | ||
| + | ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K Part:BBa_K1104246])]] | ||
| + | Promoter testing of ahpC promoter ([https://parts.igem.org/Part:BBa_K362001 Part:K362001]) and its improvements. | ||
[[File: NYMU_AA.png|frame|center]] | [[File: NYMU_AA.png|frame|center]] | ||
| + | [[File: NYMU_AAAA.png|frame|center]] | ||
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==Discussion== | ==Discussion== | ||
Latest revision as of 21:57, 27 October 2013
This part: AhpCpD1 is one of the improvement versions of BioBrick Part: ahpC promoter (Part:K362001) designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team]. AhpCpD1 (Part:BBa_K1104206) was used as sensor to ROS (Reactive Oxygen Species). More details about ROS-induced promoters and part improvements of ahpC promoter are in page:[http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].
We use the device: AhpCpD1 +E0840 (Part:BBa_K1104246) includes promoter (Part:BBa_K1104206) and Part:BBa_E0840, induce the promoter by different concentrations of H2O2, and identify the strength. After AhpCpD1 (Part:BBa_K1104206) is activated, GFP generator BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output the fluorescent protein GFP.
We tested the improved promoter: AhpCpD1 by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.
Results
This is the relative promoter strength of BBa_K1104206 under different H202 concentrations tested using BBa_K11042046 (BBa_K1104206 +E0840) and found that there is a trend where the higher the H202 concentration, the higher the promoter expression:
Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:
Promoter testing of ahpC promoter (Part:K362001) and its improvements.
Discussion
Applications of BBa_K1104206
User Reviews
UNIQ16498b1aa4fa06f1-partinfo-00000000-QINU UNIQ16498b1aa4fa06f1-partinfo-00000001-QINU