Difference between revisions of "Part:BBa K1104202:Experience"
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We tested the promoter: HemHp by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols]. This wiki page shows the protocols we used for promoter testing.
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HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104202 Part:BBa_K1104202]) is an OxyR-induced promoter, and was used as sensor to ROS(Reactive Oxygen Species).
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[[File: NYMU_42.png|frame|center|'''HemH promoter +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242])''']]
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We designed the device: HemH promoter +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242]) includes HemH promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induced HemH promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identified the strength.
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In this device, after HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104202 Part:BBa_K1104202]) is activated, GFP generator [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840] takes as input a transcriptional signal (PoPS) and produce as output GFP (green fluorescent protein). We induced HemH promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and measured the expression of GFP, and identified the strength.  
 
==Results==
 
==Results==
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Here is our result of HemHp testing:
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This is the relative promoter strength of BBa_K1104202 under different H<sub>2</sub>0<sub>2</sub> concentrations tested using BBa_K11042042 (BBa_K1104202 +E0840) and found no significant trend where the higher the H<sub>2</sub>0<sub>2</sub> concentration, the higher the promoter expression:
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[[File: NYMU_HemHp_strength.png|frame|center]]
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[[File: NYMU_all1.png|frame|center]]
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[[File: hem.png|frame|center|(Error bars represent the 95% confidence interval)]]
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Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:
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[[File: NYMU_all1.png|frame|center|Comparison between ahpC and its derived promoters.(Error bars represent the 95% confidence interval)]]  
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More details about ROS-induced promoters are in page: [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae''].
  
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==Discussion==
 
  
 
===Applications of BBa_K1104202===
 
===Applications of BBa_K1104202===

Latest revision as of 21:44, 27 October 2013


HemHp (Part:BBa_K1104202) is an OxyR-induced promoter, and was used as sensor to ROS(Reactive Oxygen Species).

HemH promoter +E0840 (Part:BBa_K1104242)

We designed the device: HemH promoter +E0840 (Part:BBa_K1104242) includes HemH promoter (Part:BBa_K1104242) and Part:BBa_E0840, induced HemH promoter by different concentrations of H2O2, and identified the strength.

In this device, after HemHp (Part:BBa_K1104202) is activated, GFP generator Part:BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output GFP (green fluorescent protein). We induced HemH promoter by different concentrations of H2O2, and measured the expression of GFP, and identified the strength.

Results

This is the relative promoter strength of BBa_K1104202 under different H202 concentrations tested using BBa_K11042042 (BBa_K1104202 +E0840) and found no significant trend where the higher the H202 concentration, the higher the promoter expression:

(Error bars represent the 95% confidence interval)

Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:

Comparison between ahpC and its derived promoters.(Error bars represent the 95% confidence interval)

More details about ROS-induced promoters are in page: [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].


Applications of BBa_K1104202

User Reviews

UNIQf8c8d18bd1ce28f1-partinfo-00000000-QINU UNIQf8c8d18bd1ce28f1-partinfo-00000001-QINU