Difference between revisions of "Part:BBa K1104202:Experience"

Latest revision as of 21:44, 27 October 2013

HemHp (Part:BBa_K1104202) is an OxyR-induced promoter, and was used as sensor to ROS(Reactive Oxygen Species).

HemH promoter +E0840 (Part:BBa_K1104242)

We designed the device: HemH promoter +E0840 (Part:BBa_K1104242) includes HemH promoter (Part:BBa_K1104242) and Part:BBa_E0840, induced HemH promoter by different concentrations of H₂O₂, and identified the strength.

In this device, after HemHp (Part:BBa_K1104202) is activated, GFP generator Part:BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output GFP (green fluorescent protein). We induced HemH promoter by different concentrations of H₂O₂, and measured the expression of GFP, and identified the strength.

Results

This is the relative promoter strength of BBa_K1104202 under different H₂0₂ concentrations tested using BBa_K11042042 (BBa_K1104202 +E0840) and found no significant trend where the higher the H₂0₂ concentration, the higher the promoter expression:

(Error bars represent the 95% confidence interval)

Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:

Comparison between ahpC and its derived promoters.(Error bars represent the 95% confidence interval)

More details about ROS-induced promoters are in page: [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].

Applications of BBa_K1104202

User Reviews

UNIQ3fbbd6421927f0a5-partinfo-00000000-QINU UNIQ3fbbd6421927f0a5-partinfo-00000001-QINU

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 __NOTOC__
-We tested the promoter: HemHp by following these [http://2013.igem.org/wiki/index.php?title=Team:NYMU-Taipei/Experiments/Protocols&oldid=243384 Promoter Testing protocols].  This wiki page shows the protocols we used for promoter testing.
+HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104202 Part:BBa_K1104202]) is an OxyR-induced promoter, and was used as sensor to ROS(Reactive Oxygen Species).
+[[File: NYMU_42.png|frame|center|'''HemH promoter +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242])''']]
+We designed the device: HemH promoter +E0840 ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242]) includes HemH promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104242 Part:BBa_K1104242]) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840], induced HemH promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and identified the strength.
+In this device, after HemHp ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K1104202 Part:BBa_K1104202]) is activated, GFP generator [https://parts.igem.org/wiki/index.php?title=Part:BBa_E0840 Part:BBa_E0840] takes as input a transcriptional signal (PoPS) and produce as output GFP (green fluorescent protein). We induced HemH promoter by different concentrations of H<sub>2</sub>O<sub>2</sub>, and measured the expression of GFP, and identified the strength.
+==Results==
+This is the relative promoter strength of BBa_K1104202 under different H<sub>2</sub>0<sub>2</sub> concentrations tested using BBa_K11042042 (BBa_K1104202 +E0840) and found no significant trend where the higher the H<sub>2</sub>0<sub>2</sub> concentration, the higher the promoter expression:
+[[File: hem.png|frame|center|(Error bars represent the 95% confidence interval)]]
+Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:
+[[File: NYMU_all1.png|frame|center|Comparison between ahpC and its derived promoters.(Error bars represent the 95% confidence interval)]]
+More details about ROS-induced promoters are in page: [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing ''Nosema ceranae''].
-Here is our result of HemHp testing:
-[[File: NYMU_HemHp_strength.png|frame|center]]
 ===Applications of BBa_K1104202===