Difference between revisions of "Part:BBa K1041002:Design"

(→‎Source)
 
(2 intermediate revisions by one other user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1041002 short</partinfo>
 
<partinfo>BBa_K1041002 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Team NRP-UEA_Norwich 2013 created this part using biobricks BBa_K1041000 and BBa_K1041001. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated in front of the AntG promoter of BBa_K1041001 to create a new biobrick.
+
Team NRP-UEA_Norwich 2013 created this part using biobricks BBa_K1041000 and BBa_K1041001. These biobricks both contain an Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated downstream of the AntG promoter of BBa_K1041001 to create this new biobrick.
  
 
===Source===
 
===Source===
−
This part was designed by our team to aid our project in identifying new strains of Antimycin-Producing Actinomycetes.The 14 known biosynthetic gene clusters contain four operons: antAB, antCDE, antFG and antHIJKLMNO. The antA gene encodes a unique ECF RNA polymerase sigma factor, referred to as σAntA, which has the sole function of regulating antimycin synthesis by activating transcription of the antFG and antHIJKLMNO genes [1].  
+
This part was designed by our team to aid our project in identifying new strains of Antimycin-Producing Actinomycetes. The 14 known antimycin biosynthetic gene clusters contain four operons: antAB, antCDE, antFG and antHIJKLMNO. The antA gene encodes a unique ECF RNA polymerase sigma factor, referred to as σAntA, which has the sole function of regulating antimycin synthesis by activating transcription of the antFG and antHIJKLMNO genes [1].  
 
Homologues of the AntA sigma factor, the key regulatory protein in antimycin biosynthesis, are present in all known gene clusters [2]. Due to this property a biosensor was designed with the AntA-regulated promoter (antGp) controlling the expression of the reporter neomycin resistance gene.
 
Homologues of the AntA sigma factor, the key regulatory protein in antimycin biosynthesis, are present in all known gene clusters [2]. Due to this property a biosensor was designed with the AntA-regulated promoter (antGp) controlling the expression of the reporter neomycin resistance gene.
−
This part was produced by performing restriction digests of the parts <partinfo>BBa_K1041000</partinfo> <partinfo>BBa_K1041001</partinfo>.
+
This part was produced by performing restriction digests of the parts <partinfo>BBa_K1041000</partinfo> and <partinfo>BBa_K1041001</partinfo>.
  
 
===References===
 
===References===
 +
1.Speike, R., Barke, J., Brearley, C., Hill, L., Yu, D., Goss, R & Hutchings, M (2011) A single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex Octospinosus, PlosOne, Volume: 6, Issue: 8.
 +
<br>
 +
2.Sandy, M., Rui, Z., Gallagher, J & Zhang, W (2012) Enzymatic Synthesis of the Dilactone Scaffold of Antimycins, American Chemical Society

Latest revision as of 22:15, 4 October 2013

AntG Promoter + RFP Coding Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 683
    Illegal AgeI site found at 795
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Team NRP-UEA_Norwich 2013 created this part using biobricks BBa_K1041000 and BBa_K1041001. These biobricks both contain an Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The RFP coding gene was excised from BBa_K1041000 and ligated downstream of the AntG promoter of BBa_K1041001 to create this new biobrick.

Source

This part was designed by our team to aid our project in identifying new strains of Antimycin-Producing Actinomycetes. The 14 known antimycin biosynthetic gene clusters contain four operons: antAB, antCDE, antFG and antHIJKLMNO. The antA gene encodes a unique ECF RNA polymerase sigma factor, referred to as σAntA, which has the sole function of regulating antimycin synthesis by activating transcription of the antFG and antHIJKLMNO genes [1]. Homologues of the AntA sigma factor, the key regulatory protein in antimycin biosynthesis, are present in all known gene clusters [2]. Due to this property a biosensor was designed with the AntA-regulated promoter (antGp) controlling the expression of the reporter neomycin resistance gene. This part was produced by performing restriction digests of the parts BBa_K1041000 and BBa_K1041001.

References

1.Speike, R., Barke, J., Brearley, C., Hill, L., Yu, D., Goss, R & Hutchings, M (2011) A single Streptomyces Symbiont Makes Multiple Antifungals to Support the Fungus Farming Ant Acromyrmex Octospinosus, PlosOne, Volume: 6, Issue: 8.
2.Sandy, M., Rui, Z., Gallagher, J & Zhang, W (2012) Enzymatic Synthesis of the Dilactone Scaffold of Antimycins, American Chemical Society