Difference between revisions of "Part:BBa K1088000:Design"
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We performed 4 silent site directed mutageneses to remove 2 EcoR1 and 2 Pst1 restriction sites | We performed 4 silent site directed mutageneses to remove 2 EcoR1 and 2 Pst1 restriction sites | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
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Latest revision as of 11:00, 2 October 2013
1-deoxyxylulose-5-phosphate synthase (Dxs) enzyme from MEP pathway isolated from B. subtilis 168
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
Illegal PstI site found at 1121
Illegal PstI site found at 1567 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
Illegal PstI site found at 1121
Illegal PstI site found at 1567 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
Illegal PstI site found at 1121
Illegal PstI site found at 1567 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1063
Illegal EcoRI site found at 1720
Illegal PstI site found at 1121
Illegal PstI site found at 1567
Illegal NgoMIV site found at 1020
Illegal AgeI site found at 913 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 862
Illegal SapI.rc site found at 1561
Design Notes
we changed TTG start codon to ATG to improve translation efficiency. We performed 4 silent site directed mutageneses to remove 2 EcoR1 and 2 Pst1 restriction sites
Source
B. subtilis 168
References
