Difference between revisions of "Part:BBa K1045001:Design"

Latest revision as of 18:08, 17 October 2013

DarR ORF with inversed Pre- and Suffix

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 581
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 594
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 31
1000
COMPATIBLE WITH RFC[1000]

Design Notes

In order to express DarR in Escherichia coli, the GTG-start codon was mutated to an ATG. Also, the first few codons were changed according to codon usage in E. coli via mutagenesis PCR (with the forward primer).

Source

This part was amplified from the genomic DNA of Mycobacterium smegmatis purchased from DSMZ ([http://www.dsmz.de/catalogues/details/culture/DSM-43756.html?tx_dsmzresources_pi5%5BreturnPid%5D=304 DSM No. 43756]).

References

Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096

@@ Line 10: / Line 10: @@
 ===Source===
-This part was amplified from the genomic DNA of ''Mycobacterium smegmatis'' MC2 155.
+This part was amplified from the genomic DNA of ''Mycobacterium smegmatis'' purchased from DSMZ ([http://www.dsmz.de/catalogues/details/culture/DSM-43756.html?tx_dsmzresources_pi5%5BreturnPid%5D=304 DSM No. 43756]).
 ===References===
 Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in ''Mycobacterium smegmatis''”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096