Difference between revisions of "Part:BBa K1041003"

Latest revision as of 22:30, 4 October 2013

Neomycin Resistance Reporter Gene

Team NRP-UEA_Norwich 2013 desgined this part using biobricks BBa_K1041000 and BBa_K1041001. These biobricks both contain an Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene would be excised from BBa_K1041001 and ligated downstream of the promoter of BBa_K1041000 to create a new biobrick. When prepared, the resulting biobrick should provide resistance to neomycin (kanamycin) when transformed into any strain of E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 855
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 704
Illegal SapI.rc site found at 914

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1041003 short</partinfo>
-Team NRP-UEA_Norwich 2013 desgined this part using biobricks <partinfo>BBa_K1041000</partinfo> and <partinfo>BBa_K1041002</partinfo>. These biobricks both contain a Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene was excised from BBa_K1041002 and ligated in front of the promoter of BBa_K1041000 to create a new biobrick.
+Team NRP-UEA_Norwich 2013 desgined this part using biobricks <partinfo>BBa_K1041000</partinfo> and <partinfo>BBa_K1041001</partinfo>. These biobricks both contain an Nde1 site after their promoter sequence, enabling a restriction digest to be performed. The Neomycin resistance coding gene would be excised from BBa_K1041001 and ligated downstream of the promoter of BBa_K1041000 to create a new biobrick. When prepared, the resulting biobrick should provide resistance to neomycin (kanamycin) when transformed into any strain of ''E. coli''.
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