Difference between revisions of "Part:BBa K1065103"

Latest revision as of 18:25, 3 October 2013

PchBA

This BioBrick contains the coding sequence for the two enzymes PchA and PchB. This part was submitted in pSB1C3 to the registry by the UniTN-Trento 2013, using an original part of iGEM MIT 2006 BBa_J45319 in pSB1AT3.

This part was cloned by the iGEM UniTN Trento 2013 team for the creation of Wintergreen Devices BBa_K1065102 and BBa_K1065106.

Usage and Biology

PchB is a isochorismate-pyruvate lyase while PchA is a isochorismate synthase, both from the microorganism Pseudomonas aeruginosa. These enzymes are responsible for the production of salicylate acid.

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Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 326
Illegal NgoMIV site found at 730
Illegal NgoMIV site found at 855
Illegal NgoMIV site found at 866
Illegal NgoMIV site found at 1147
Illegal NgoMIV site found at 1615
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 This BioBrick contains the coding sequence for the two enzymes PchA and PchB.
-This part was submitted in pSB1C3 to the registry the UniTN-Trento 2013, using an original part of iGEM MIT 2006 <partinfo>BBa_J45319</partinfo> in pSB1AT3.
+This part was submitted in pSB1C3 to the registry by the UniTN-Trento 2013, using an original part of iGEM MIT 2006 <partinfo>BBa_J45319</partinfo> in pSB1AT3.<br>
+This part was cloned by the iGEM UniTN Trento 2013 team for the creation of Wintergreen Devices <partinfo>BBa_K1065102</partinfo> and <partinfo>BBa_K1065106</partinfo>.
 ===Usage and Biology===
@@ Line 14: / Line 16: @@
 </html>
+<!-- -->
+<span class='h3bb'>Sequence and Features</span>
+<partinfo>BBa_K1065103 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display