Difference between revisions of "Part:BBa K1065309:Design"
(→References) |
(→References) |
||
| (One intermediate revision by one other user not shown) | |||
| Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
We designed this part by inserting the part <partinfo>BBa_K1065302</partinfo> uptsream <partinfo>BBa_K1065002</partinfo>. So we built a part consisting of the blue light circuit that regulates amilCP+EFE production. | We designed this part by inserting the part <partinfo>BBa_K1065302</partinfo> uptsream <partinfo>BBa_K1065002</partinfo>. So we built a part consisting of the blue light circuit that regulates amilCP+EFE production. | ||
| − | Note that a terminator is missing | + | Note that a terminator is missing before PfixK2. We suggest to instert at least one terminator before the promoter in order to have a better defined switch from the ON state to the OFF state. |
===Source=== | ===Source=== | ||
| Line 20: | Line 20: | ||
2) Ohlendorf, R., Vidavski, R.R., Eldar, A., Moffat, K. & Möglich, A.(2012). From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. J. Mol. Biol., 416: 534: 542<br> | 2) Ohlendorf, R., Vidavski, R.R., Eldar, A., Moffat, K. & Möglich, A.(2012). From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. J. Mol. Biol., 416: 534: 542<br> | ||
3) Nagahama K, Ogawa T, Fujii T, Tazaki M, Tanase S, et al. (1991) Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2. Journal of General Microbiology 137: 2281–2286.<br> | 3) Nagahama K, Ogawa T, Fujii T, Tazaki M, Tanase S, et al. (1991) Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2. Journal of General Microbiology 137: 2281–2286.<br> | ||
| − | Fukuda H, Ogawa T, Ishihara K, Fujii T, Nagahama K, et al. (1992) Molecular cloning in Escherichia coli, expression, and nucleotide sequence of the gene for the ethylene-forming enzyme of Pseudomonas syringae pv. phaseolicola PK2. Biochem Biophys Res Commun 188: 826–832.<br> | + | 4) Fukuda H, Ogawa T, Ishihara K, Fujii T, Nagahama K, et al. (1992) Molecular cloning in Escherichia coli, expression, and nucleotide sequence of the gene for the ethylene-forming enzyme of Pseudomonas syringae pv. phaseolicola PK2. Biochem Biophys Res Commun 188: 826–832.<br> |
| − | + | 5) Guerrero F, Carbonell. V., Cossu M, Correddu D, Jones PR (2012) Ethylene Synthesis and Regulated Expression of Recombinant Protein in Synechocystis sp. PCC 6803. PLoS ONE 7(11): e50470. | |
Latest revision as of 23:10, 4 October 2013
light regulated circuit producing amilGFP+EFE at dark
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3309
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 770
Illegal NgoMIV site found at 842
Illegal NgoMIV site found at 932
Illegal NgoMIV site found at 950
Illegal NgoMIV site found at 1462
Illegal NgoMIV site found at 1755
Illegal NgoMIV site found at 1849
Illegal AgeI site found at 484
Illegal AgeI site found at 1630
Illegal AgeI site found at 4060 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1519
Illegal BsaI.rc site found at 383
Design Notes
We designed this part by inserting the part BBa_K1065302 uptsream BBa_K1065002. So we built a part consisting of the blue light circuit that regulates amilCP+EFE production. Note that a terminator is missing before PfixK2. We suggest to instert at least one terminator before the promoter in order to have a better defined switch from the ON state to the OFF state.
Source
The part sources are:
BBa_K1065302 (the improved version of the part BBa_K952003);
BBa_K1065002 (EFE+terminators)
References
1) Moglich A, Ayers RA and Moffat K. (2009) Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Bio. 385, 5, 1433-1444.
2) Ohlendorf, R., Vidavski, R.R., Eldar, A., Moffat, K. & Möglich, A.(2012). From Dusk till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression. J. Mol. Biol., 416: 534: 542
3) Nagahama K, Ogawa T, Fujii T, Tazaki M, Tanase S, et al. (1991) Purification and properties of an ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2. Journal of General Microbiology 137: 2281–2286.
4) Fukuda H, Ogawa T, Ishihara K, Fujii T, Nagahama K, et al. (1992) Molecular cloning in Escherichia coli, expression, and nucleotide sequence of the gene for the ethylene-forming enzyme of Pseudomonas syringae pv. phaseolicola PK2. Biochem Biophys Res Commun 188: 826–832.
5) Guerrero F, Carbonell. V., Cossu M, Correddu D, Jones PR (2012) Ethylene Synthesis and Regulated Expression of Recombinant Protein in Synechocystis sp. PCC 6803. PLoS ONE 7(11): e50470.