Difference between revisions of "Part:BBa K1065300"
 
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This part is composed by a pLac and <partinfo>K952003</partinfo>. The part does not work because K952003 lacks the RBS between the Pfixk2 and the amilGFP.<br>
 
This part is composed by a pLac and <partinfo>K952003</partinfo>. The part does not work because K952003 lacks the RBS between the Pfixk2 and the amilGFP.<br>
 
We improved K952003 with the addition of a RBS before the amilGFP by mutagenesis.<BR>The new resulting part is named <partinfo>K1065305</partinfo>. We also created the part <partinfo>K1065302</partinfo> that contains pLac and <partinfo>K1065305</partinfo>.
 
We improved K952003 with the addition of a RBS before the amilGFP by mutagenesis.<BR>The new resulting part is named <partinfo>K1065305</partinfo>. We also created the part <partinfo>K1065302</partinfo> that contains pLac and <partinfo>K1065305</partinfo>.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 13:20, 28 September 2013

R0010-YF1-FixJ-PfixK2-amilGFP

This part is composed by a pLac and BBa_K952003. The part does not work because K952003 lacks the RBS between the Pfixk2 and the amilGFP.
We improved K952003 with the addition of a RBS before the amilGFP by mutagenesis.
The new resulting part is named BBa_K1065305. We also created the part BBa_K1065302 that contains pLac and BBa_K1065305.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 842
    Illegal NgoMIV site found at 932
    Illegal NgoMIV site found at 950
    Illegal NgoMIV site found at 1462
    Illegal NgoMIV site found at 1755
    Illegal NgoMIV site found at 1849
    Illegal AgeI site found at 484
    Illegal AgeI site found at 1630
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1519
    Illegal BsaI.rc site found at 383