Difference between revisions of "Part:BBa K1065306"
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This is an intermediate part to build a blue light inducible genetic circuit. The part produces YF1 and FixJ under the control of a strong promoter of the Anderson family. | This is an intermediate part to build a blue light inducible genetic circuit. The part produces YF1 and FixJ under the control of a strong promoter of the Anderson family. | ||
+ | This part was used to create a complete blue light circuit <partinfo>BBa_K1065310</partinfo> that was fully characterized by iGEM UniTN Trento 2013. | ||
Note that this part is in pSB1A2 and it was not submitted to the registry. If you are interested in this part please contact iGEM UniTN Trento 2013. | Note that this part is in pSB1A2 and it was not submitted to the registry. If you are interested in this part please contact iGEM UniTN Trento 2013. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 08:32, 30 September 2013
J23100+YF1+FixJ
This is an intermediate part to build a blue light inducible genetic circuit. The part produces YF1 and FixJ under the control of a strong promoter of the Anderson family. This part was used to create a complete blue light circuit BBa_K1065310 that was fully characterized by iGEM UniTN Trento 2013.
Note that this part is in pSB1A2 and it was not submitted to the registry. If you are interested in this part please contact iGEM UniTN Trento 2013.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 605
Illegal NgoMIV site found at 677
Illegal NgoMIV site found at 767
Illegal NgoMIV site found at 785
Illegal NgoMIV site found at 1297
Illegal NgoMIV site found at 1590
Illegal NgoMIV site found at 1684
Illegal AgeI site found at 319
Illegal AgeI site found at 1465 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1354
Illegal BsaI.rc site found at 218