Difference between revisions of "Part:BBa K1129012"

Latest revision as of 07:42, 22 October 2017

T7 Repeat/Spacer Region of T7 Bacteriophage

T7 Repeat/Spacer Region of Streptococcus thermophilus

This part confers resistance against T7 phage upon a strain containing the Cas9 and TracrRNA parts.

This part is computationally verified to target all of 13a, K30, T3 and T7 genomes

Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] By associating with CAS9, an enzyme with both endonuclease and DNA-binding activity, CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner similar to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR Sequence and Features

New application

Group: SCUT-FSE-CHINA 2017

The N20 sequence 5'-ACCAATCTGAACCACTGTGT-3‘ has been selected from BBa K1129012 to form a new sgRNA in our CRISPR system. The new sgRNA has been submitted in part BBa_K2325003.

We fused crRNA and tracrRNA as a single synthetic guide RNA (sgRNA), while UBC2013 constructed crRNA (repeat-spacer array) and tracrRNA separately. In their construct, the crRNA and tracrRNA were separately transcript, followed by the combination of these transcripts to form the sgRNA. The resist efficiency may decrease when the crRNA and tracrRNA not 100% match successfully. The way we constructed the sgRNA obviated the need for processing the transcribed CRISPR array (pre-crRNA) into individual crRNA components, helping to promote the function of resistance against T7 phage.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 This part is computationally verified to target all of 13a, K30, T3 and T7 genomes
-Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms.
+Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] By associating with CAS9, an enzyme with both endonuclease and DNA-binding activity, CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner similar to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR Sequence and Features
-http://en.wikipedia.org/wiki/CRISPR
+===New application===
+Group: <b>SCUT-FSE-CHINA 2017</b>
+The N20 sequence 5'-ACCAATCTGAACCACTGTGT-3‘ has been selected from BBa K1129012 to form a new sgRNA in our CRISPR system. The new sgRNA has been submitted in part <partinfo>BBa_K2325003</partinfo>.
+We fused crRNA and tracrRNA as a single synthetic guide RNA (sgRNA), while UBC2013 constructed crRNA (repeat-spacer array) and tracrRNA separately. In their construct, the crRNA and tracrRNA were separately transcript, followed by the combination of these transcripts to form the sgRNA. The resist efficiency may decrease when the crRNA and tracrRNA not 100% match successfully. The way we constructed the sgRNA obviated the need for processing the transcribed CRISPR array (pre-crRNA) into individual crRNA components, helping to promote the function of resistance against T7 phage.