Difference between revisions of "Part:BBa K1129007"

Latest revision as of 23:19, 27 October 2013

T4 Repeat/Spacer Region of T4 Bacteriophage

Repeat/Spacer Region Confers resistance to T4 page upon those strains containing Cas9 and TracrRNA.

This spacer is computationally verified to targets all of AR1 HX01 ime09 RB14 RB32 RB51 RB69 T4 vB_EcoM_ACG-C40 wV7 phage genomes.

Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] By associating with CAS9, an enzyme with both endonuclease and DNA-binding activity, CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner similar to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 AR1 HX01 ime09 RB14 RB32 RB51 RB69 T4 vB_EcoM_ACG-C40 wV7 phage genomes.
-Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner analogous to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR
+Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] By associating with CAS9, an enzyme with both endonuclease and DNA-binding activity, CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner similar to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR
 <!-- Add more about the biology of this part here
 ===Usage and Biology===