Difference between revisions of "Part:BBa K1230007:Experience"

Latest revision as of 22:09, 5 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1230007

User Reviews

UNIQ249f5bc840940ccd-partinfo-00000000-QINU UNIQ249f5bc840940ccd-partinfo-00000001-QINU

@@ Line 23: / Line 23: @@
 From each tube we did 8 decimal dilutions in 1.5 Eppendorf tubes with LB. These are the results.
-https://static.igem.org/mediawiki/2013/a/a1/Cienciasunam_C1.png
-https://static.igem.org/mediawiki/2013/b/be/Cienciasunam_C2.png
 As we can see here, there is no basal level transcription on this experiment. So the problem in theirs characterisation may be due to the codon usage they used.
@@ Line 31: / Line 28: @@
 For more information on the characterisation, visit our notebook on our wiki http://2013.igem.org/Team:Ciencias-UNAM/Project/WetLab/Protocols
+We successfully cloned LL-37 with E. coli codon usage,designed by Team Trieste 2008 with a different promoter, pBad, but we could not observed a reduction in the viability with it.
+We performed several trials to obtain cells that express LL-37 with human codon usage with no results; this might be due to the toxicity of the peptide itself. We intend to co-express LL-37 with the GFP generator too.
 |};
 <!-- End of the user review template -->
 <!-- DON'T DELETE --><partinfo>BBa_K1230007 EndReviews</partinfo>