Difference between revisions of "Part:BBa K1166001"
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<partinfo>BBa_K1166001 short</partinfo> | <partinfo>BBa_K1166001 short</partinfo> | ||
+ | This part includes the native expression cassette for FNR. | ||
− | FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under | + | FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under hypixia FNR is in its active state and is found as a homodimer. When O2 is present FNR is converted into a monomer which is no longer active (Reinhart, et al., 2008). |
Therefore, co-expression of FNR with genes regulated by hypoxic promoters bearing a FNR binding site would help to have a more sensitive hypoxic response. Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein. | Therefore, co-expression of FNR with genes regulated by hypoxic promoters bearing a FNR binding site would help to have a more sensitive hypoxic response. Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein. | ||
[[File:FNR-nirB_characterization.jpg|800px|]] | [[File:FNR-nirB_characterization.jpg|800px|]] | ||
− | The graphic shows the effect of FNR in the promoter nirB. When GFP is expressed under the regulation of nirB and without FNR (pNG) the same basal fluorescence is detected through | + | The graphic shows the effect of FNR (K1166000) in the promoter nirB (BBa_K905000). When GFP is expressed under the regulation of nirB (BBa_K905000) and without FNR (pNG=promoter nirb + GFP) the same basal fluorescence is detected through all oxygen levels, on the other hand, when FNR is co-expressed (pFG=FNR, promoter nirb+GFP), a rise in fluorescence is observed at hypoxic conditions. |
==References== | ==References== |
Latest revision as of 17:38, 4 October 2013
Fumarate-Nitrate Reductase This part includes the native expression cassette for FNR.
FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under hypixia FNR is in its active state and is found as a homodimer. When O2 is present FNR is converted into a monomer which is no longer active (Reinhart, et al., 2008). Therefore, co-expression of FNR with genes regulated by hypoxic promoters bearing a FNR binding site would help to have a more sensitive hypoxic response. Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein.
The graphic shows the effect of FNR (K1166000) in the promoter nirB (BBa_K905000). When GFP is expressed under the regulation of nirB (BBa_K905000) and without FNR (pNG=promoter nirb + GFP) the same basal fluorescence is detected through all oxygen levels, on the other hand, when FNR is co-expressed (pFG=FNR, promoter nirb+GFP), a rise in fluorescence is observed at hypoxic conditions.
References
Reinhart F, Achebach S, Koch T, Unden G. (2008). Reduced apo-fumarate nitrate reductase regulator (apoFNR) as the major form of FNR in aerobically growing Escherichia coli. J Bacteriol. 190(3):879-86.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]