Difference between revisions of "Part:BBa K1021002:Experience"

(Applications of BBa_K1021002)
 
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Cornell iGEM characterized a composite of this part, BBa_K1021033, in <i>E. coli</i> BL21-A1 using a zone of inhibition test. The data is displayed below; at concentrations of 200 and 2000 μg/mL, the wild-type strain was significantly more inhibited than the allegedly hygromycin-resistant strain (one-tailed student's t-test, α=0.005).  
 
Cornell iGEM characterized a composite of this part, BBa_K1021033, in <i>E. coli</i> BL21-A1 using a zone of inhibition test. The data is displayed below; at concentrations of 200 and 2000 μg/mL, the wild-type strain was significantly more inhibited than the allegedly hygromycin-resistant strain (one-tailed student's t-test, α=0.005).  
 
[[File:Hph.png]]
 
[[File:Hph.png]]
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*This part is intended to be used as a selective marker for genetic engineering of fungi.
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*Hygromycin is effective against bacteria as well, so the part can be used in a greater variety of organisms.
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 02:00, 29 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1021002

Cornell iGEM characterized a composite of this part, BBa_K1021033, in E. coli BL21-A1 using a zone of inhibition test. The data is displayed below; at concentrations of 200 and 2000 μg/mL, the wild-type strain was significantly more inhibited than the allegedly hygromycin-resistant strain (one-tailed student's t-test, α=0.005). Hph.png

  • This part is intended to be used as a selective marker for genetic engineering of fungi.
  • Hygromycin is effective against bacteria as well, so the part can be used in a greater variety of organisms.

User Reviews

UNIQ9ea3bf93d2a8fe79-partinfo-00000000-QINU UNIQ9ea3bf93d2a8fe79-partinfo-00000001-QINU