Difference between revisions of "Part:BBa K1026009:Design"
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So we put the G-box onto this exact region, between -35 region and -10 region of the original promoter. | So we put the G-box onto this exact region, between -35 region and -10 region of the original promoter. | ||
Therefore, we expect that activated CcaR will bind and hinder RNA polymerase binding, and thus prevents transcription initialization. | Therefore, we expect that activated CcaR will bind and hinder RNA polymerase binding, and thus prevents transcription initialization. | ||
| − | + | [[File:SJTU RationallyDesignedGreenPromoter.jpg]] | |
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===Source=== | ===Source=== | ||
Latest revision as of 01:53, 28 September 2013
A rationally designed promoter negative regulated by CcaR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 42
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We discovered a segment around the -35 region of BBa_J23107 that is almost identical to G-box, the binding region of CcaR on PcpcG2.
So we put the G-box onto this exact region, between -35 region and -10 region of the original promoter.
Therefore, we expect that activated CcaR will bind and hinder RNA polymerase binding, and thus prevents transcription initialization.
Source
Synechocystissp. PCC 6803