Difference between revisions of "Part:BBa K1019000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The fimE acts opposite to fimB by mediating the '''on to off''' switching of the fim switch/promoter, fimS, which allows fimA (and associated fim operon genes) to be expressed depending on its state. Both fimB and fimE are recombinases that catalyze the inversion of fimS, thus controlling the expression of fimbriae. | + | The fimE gene acts opposite to fimB by mediating the '''on to off''' switching of the fim switch/promoter, fimS, which allows fimA (and associated fim operon genes) to be expressed depending on its state. Both fimB and fimE are recombinases that catalyze the inversion of fimS, thus controlling the expression of fimbriae. |
| − | Overexpression of fimB should in principle encourage fimbriae formation, thus leading to increased adherence to cells and surfaces. | + | Overexpression of fimB should in principle encourage fimbriae formation, thus leading to increased adherence to cells and surfaces. Fimbriae expression constitutes a defining feature of biofilms. |
For further information please refer to [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10309 EcoCyc]. | For further information please refer to [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10309 EcoCyc]. | ||
Latest revision as of 03:25, 28 September 2013
FimB: positive regulator of type I fimbriae
The fimB gene encodes for the FimB protein, a transcriptional regulator which mediates off to on switching of the major type 1 subunit fimbrin (pilin) FimA and other fimbrial genes in the fim operon.
Usage and Biology
The fimE gene acts opposite to fimB by mediating the on to off switching of the fim switch/promoter, fimS, which allows fimA (and associated fim operon genes) to be expressed depending on its state. Both fimB and fimE are recombinases that catalyze the inversion of fimS, thus controlling the expression of fimbriae.
Overexpression of fimB should in principle encourage fimbriae formation, thus leading to increased adherence to cells and surfaces. Fimbriae expression constitutes a defining feature of biofilms.
For further information please refer to [http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10309 EcoCyc].
References
Escherichia coli K-12 substr. MG1655 Polypeptide: regulator for fimA. (2013). Ecocyc. Retrieved May 26, 2013, from http://www.ecocyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10309
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]