Difference between revisions of "Part:BBa K1189024:Experience"
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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===Applications of BBa_K1189024===
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<h1>Light chain human ferritin</h1>
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This part used primarily for the construction of parts containing the light ferritin subunit (<partinfo>BBa_K1189020</partinfo> <partinfo>BBa_K1189018</partinfo>).
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<p>This part is the light ferritin subunit from human ferritin, inspired by <a href="http://www.uniprot.org/uniprot/P02792">P02792 [UniParc]</a>. Ferritin is ubiquitous across prokaryotic and eukaryotic systems and is used to buffer intracellular iron. This part, along with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189025">heavy ferritin subunit</a>, form a 24 multimeric iron sequestering nanoparticle (Chasteen <i>et al.</i>, 1991). The light ferritin purportedly contributes to nucleation to initiate iron core formation in ferritin molecules (Chasteen <i>et al.</i>, 1999). These nanoparticles are robust, remain stable at extreme pHs, and withstand temperature variations (Kim <i>et al.</i>, 1999).
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</p>
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<figure>
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<img src="https://static.igem.org/mediawiki/2013/1/18/UCalgary2013TRFerritinrender2png.png" alt="Ferritin" width="300" height="300">
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<figcaption>
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<p><b>Figure 1.</b> Ribbon visualization of a fully assembled ferritin protein.</p>
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</figcaption>
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</figure>
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<h1>Ferritin's utility in iGEM</h1>
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<p>Ferritin as a nanoparticle is interesting for other iGEM teams for two reasons. Firstly, its iron core can be replaced with other compounds to serve different functions. The <a href="http://2013.igem.org/Team:Calgary">iGEM Calgary 2013</a> demonstrated this by chemically modifying recombinant ferritin's iron core into a <a href="http://2013.igem.org/Team:Calgary/Project/OurSensor/Reporter/PrussianBlueFerritin">robust colourmetric reporter</a>. Other intriguing applications include making ferritin’s iron core magnetically active as magnetoferritin (Jordan et al. 2013), using ferritin as a nanocage for other metals, or the incorporation of other reporters such as quantum dots (Naito et al. 2013) (Figure 2).</p>
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<figure>
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<img src="https://static.igem.org/mediawiki/2013/b/b8/UCalgary2013TRFerritincorechange.png" alt="Ferritin Core Modulation" width="800" height="400">
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<figcaption>
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<p><b>Figure 2.</b> Chemically modifying the iron core of ferritin allows ferritin to be moulded to fit a wide magnitude of applications. Additionally the ferritin subunits can act as a nanocage to encapsulate completely new cores. </p>
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</figcaption>
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</figure>
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<p>Secondly, the ferritin nanoparticle is useful for iGEM teams as a self-assembling and spherical protein scaffold. Each of the 24 subunits forming ferritin can be fused to proteins of interest, such that when the nanoparticle assembles, proteins surround the ferritin sphere (Kim et al., 2011). The <a href="http://2013.igem.org/Team:Calgary">iGEM Calgary 2013 team</a> demonstrated this by binding DNA sensing proteins, <a href="http://2013.igem.org/Team:Calgary/Project/OurSensor/Detector">TALEs</a>, as part of their <a href="http://2013.igem.org/Team:Calgary/Project/OurSensor">FerriTALE sensor</a>. The Calgary team also constructed ferritin subunits with a <a href="http://2013.igem.org/Team:Calgary/Project/OurSensor/Linker">coiled-coil linker</a> system so that other teams can scaffold proteins to E-coil ferritin (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189018">BBa_K1189018</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189019">BBa_K1189019</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189020">BBa_K1189020</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189037">BBa_K1189037</a>). See Figure 3 for a demonstration of these applications.</p>
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<figure>
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<img src="https://static.igem.org/mediawiki/2013/0/07/UCalgary2013TRCoilflexibility.png" alt="FerriTALE Scaffold Modularity" width="800" height="219" >
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<figcaption>
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<p><b>Figure 3.</b> Using the E and K coils in combination with ferritin as a scaffold system allows the creation of brand new FerriTALEs or protein scaffolds.</a></p>
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</figcaption>
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</figure>
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<h1>References</h1>
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<li>Chasteen, N. D., & Harrison, P. M. (1999). Mineralization in ferritin: an efficient means of iron storage. Journal of structural biology, 126(3), 182-194.</li>
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<li>Clavijo Jordan, V., Caplan, M. R., & Bennett, K. M. (2010). Simplified synthesis and relaxometry of magnetoferritin for magnetic resonance imaging. Magnetic Resonance in Medicine, 64(5), 1260-1266.</li>
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<li>Kim, S. E., Ahn, K. Y., Park, J. S., Kim, K. R., Lee, K. E., Han, S. S., & Lee, J. (2011). Fluorescent ferritin nanoparticles and application to the aptamer sensor. Analytical chemistry, 83(15), 5834-5843.</li>
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<li>Naito, M., Iwahori, K., Miura, A., Yamane, M., & Yamashita, I. (2010). Circularly polarized luminescent CdS quantum dots prepared in a protein nanocage. Angewandte Chemie International Edition, 49(39), 7006-7009.</li>
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<br></br>
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<h1>User Reviews</h1>
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<li>iGEM Calgary 2013: The light ferritin gene sequence as stated with this basic part was functional when expressed as C terminal fusion proteins (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189018">BBa_K1189018</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189021">BBa_K1189021</a>, <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189037">BBa_K1189037</a>) with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1189025">heavy ferritin subunit</a>. We did not explore expressibility of light ferritin chain in isolation and hope to see other teams take this on.</li>
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</html>
  
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===User Reviews===
 
 
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Latest revision as of 10:43, 30 October 2013

Light chain human ferritin

This part is the light ferritin subunit from human ferritin, inspired by P02792 [UniParc]. Ferritin is ubiquitous across prokaryotic and eukaryotic systems and is used to buffer intracellular iron. This part, along with the heavy ferritin subunit, form a 24 multimeric iron sequestering nanoparticle (Chasteen et al., 1991). The light ferritin purportedly contributes to nucleation to initiate iron core formation in ferritin molecules (Chasteen et al., 1999). These nanoparticles are robust, remain stable at extreme pHs, and withstand temperature variations (Kim et al., 1999).

Ferritin

Figure 1. Ribbon visualization of a fully assembled ferritin protein.

Ferritin's utility in iGEM

Ferritin as a nanoparticle is interesting for other iGEM teams for two reasons. Firstly, its iron core can be replaced with other compounds to serve different functions. The iGEM Calgary 2013 demonstrated this by chemically modifying recombinant ferritin's iron core into a robust colourmetric reporter. Other intriguing applications include making ferritin’s iron core magnetically active as magnetoferritin (Jordan et al. 2013), using ferritin as a nanocage for other metals, or the incorporation of other reporters such as quantum dots (Naito et al. 2013) (Figure 2).

Ferritin Core Modulation

Figure 2. Chemically modifying the iron core of ferritin allows ferritin to be moulded to fit a wide magnitude of applications. Additionally the ferritin subunits can act as a nanocage to encapsulate completely new cores.

Secondly, the ferritin nanoparticle is useful for iGEM teams as a self-assembling and spherical protein scaffold. Each of the 24 subunits forming ferritin can be fused to proteins of interest, such that when the nanoparticle assembles, proteins surround the ferritin sphere (Kim et al., 2011). The iGEM Calgary 2013 team demonstrated this by binding DNA sensing proteins, TALEs, as part of their FerriTALE sensor. The Calgary team also constructed ferritin subunits with a coiled-coil linker system so that other teams can scaffold proteins to E-coil ferritin (BBa_K1189018, BBa_K1189019, BBa_K1189020, BBa_K1189037). See Figure 3 for a demonstration of these applications.

FerriTALE Scaffold Modularity

Figure 3. Using the E and K coils in combination with ferritin as a scaffold system allows the creation of brand new FerriTALEs or protein scaffolds.

References

  • Chasteen, N. D., & Harrison, P. M. (1999). Mineralization in ferritin: an efficient means of iron storage. Journal of structural biology, 126(3), 182-194.
  • Clavijo Jordan, V., Caplan, M. R., & Bennett, K. M. (2010). Simplified synthesis and relaxometry of magnetoferritin for magnetic resonance imaging. Magnetic Resonance in Medicine, 64(5), 1260-1266.
  • Kim, S. E., Ahn, K. Y., Park, J. S., Kim, K. R., Lee, K. E., Han, S. S., & Lee, J. (2011). Fluorescent ferritin nanoparticles and application to the aptamer sensor. Analytical chemistry, 83(15), 5834-5843.
  • Naito, M., Iwahori, K., Miura, A., Yamane, M., & Yamashita, I. (2010). Circularly polarized luminescent CdS quantum dots prepared in a protein nanocage. Angewandte Chemie International Edition, 49(39), 7006-7009.


    • User Reviews

  • iGEM Calgary 2013: The light ferritin gene sequence as stated with this basic part was functional when expressed as C terminal fusion proteins (BBa_K1189018, BBa_K1189021, BBa_K1189037) with the heavy ferritin subunit. We did not explore expressibility of light ferritin chain in isolation and hope to see other teams take this on.

    • UNIQd1b41809335dda8f-partinfo-00000001-QINU UNIQd1b41809335dda8f-partinfo-00000002-QINU