Difference between revisions of "Part:BBa K1166001"

Latest revision as of 17:38, 4 October 2013

Fumarate-Nitrate Reductase This part includes the native expression cassette for FNR.

FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under hypixia FNR is in its active state and is found as a homodimer. When O2 is present FNR is converted into a monomer which is no longer active (Reinhart, et al., 2008). Therefore, co-expression of FNR with genes regulated by hypoxic promoters bearing a FNR binding site would help to have a more sensitive hypoxic response. Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein.

The graphic shows the effect of FNR (K1166000) in the promoter nirB (BBa_K905000). When GFP is expressed under the regulation of nirB (BBa_K905000) and without FNR (pNG=promoter nirb + GFP) the same basal fluorescence is detected through all oxygen levels, on the other hand, when FNR is co-expressed (pFG=FNR, promoter nirb+GFP), a rise in fluorescence is observed at hypoxic conditions.

References

Reinhart F, Achebach S, Koch T, Unden G. (2008). Reduced apo-fumarate nitrate reductase regulator (apoFNR) as the major form of FNR in aerobically growing Escherichia coli. J Bacteriol. 190(3):879-86.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1166001 short</partinfo>
+This part includes the native expression cassette for FNR.
-FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under anoxic conditions FNR is in the active state and is found as a homodimer. When O2 is present FNR is converted into a monomer which is no longer active (Reinhart, et al., 2008).
+FNR (fumarate nitrate reductase regulator) is a transcriptional regulator that functions as an O2 sensor. Under hypixia FNR is in its active state and is found as a homodimer. When O2 is present FNR is converted into a monomer which is no longer active (Reinhart, et al., 2008).
 Therefore, co-expression of FNR with genes regulated by hypoxic promoters bearing a FNR binding site would help to have a more sensitive hypoxic response.  Activity is proven by comparing the expression of GFP (BBa_E0040) by promoters with and without the simultaneous expression of the FNR protein.
+[[File:FNR-nirB_characterization.jpg|800px|]]
+The graphic shows the effect of FNR (K1166000) in the promoter nirB (BBa_K905000). When GFP is expressed under the regulation of nirB (BBa_K905000) and without FNR (pNG=promoter nirb + GFP) the same basal fluorescence is detected through all oxygen levels, on the other hand, when FNR is co-expressed (pFG=FNR, promoter nirb+GFP), a rise in fluorescence is observed at hypoxic conditions.
 ==References==