Difference between revisions of "Part:BBa K1124011:Design"

Latest revision as of 21:55, 27 September 2013

hpaBC (+RBS(natural))

Assembly Compatibility:

We cloned hpaB and hpaC gene with natural(genomic) RBS, so this part can be combined with promoter parts without adding RBS parts between them.

Note that E. coli K-12 str. have no hpaB or hpaC gene.

This part was cloned from E. coli BL21(DE3) genome by PCR. We added restriction sites on both ends of the genomic sequence.

Links to NCBI gene detabase

http://www.ncbi.nlm.nih.gov/gene/8183130 (hpaB)

http://www.ncbi.nlm.nih.gov/gene/8183129 (hpaC)

@@ Line 7: / Line 7: @@
 ===Design Notes===
-This part was cloned from ''E. coli'' BL21(DE3) genome by PCR. We added restriction sites on both ends of the genomic sequence.
+We cloned hpaB and hpaC gene with natural(genomic) RBS, so this part can be combined with promoter parts without adding RBS parts between them.
+Note that ''E. coli'' K-12 str. have no hpaB or hpaC gene.
 ===Source===
-''E. coli'' BL21(DE3)
+This part was cloned from ''E. coli'' BL21(DE3) genome by PCR. We added restriction sites on both ends of the genomic sequence.
 '''Links to NCBI gene detabase'''