Difference between revisions of "Part:BBa K1124011:Design"
 
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===Design Notes===
 
===Design Notes===
  
−
This part was cloned from ''E. coli'' BL21(DE3) genome by PCR. We added restriction sites on both ends of the genomic sequence.
+
We cloned hpaB and hpaC gene with natural(genomic) RBS, so this part can be combined with promoter parts without adding RBS parts between them.
 +
 
 +
Note that ''E. coli'' K-12 str. have no hpaB or hpaC gene.
  
 
===Source===
 
===Source===
−
''E. coli'' BL21(DE3)
+
 
 +
This part was cloned from ''E. coli'' BL21(DE3) genome by PCR. We added restriction sites on both ends of the genomic sequence.
  
 
'''Links to NCBI gene detabase'''
 
'''Links to NCBI gene detabase'''
  
−
http://www.ncbi.nlm.nih.gov/gene/8183130 (hpaC)
+
http://www.ncbi.nlm.nih.gov/gene/8183130 (hpaB)
  
−
http://www.ncbi.nlm.nih.gov/gene/8183129 (hpaB)
+
http://www.ncbi.nlm.nih.gov/gene/8183129 (hpaC)
  
 
===References===
 
===References===

Latest revision as of 21:55, 27 September 2013

hpaBC (+RBS(natural))


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 97
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1877


Design Notes

We cloned hpaB and hpaC gene with natural(genomic) RBS, so this part can be combined with promoter parts without adding RBS parts between them.

Note that E. coli K-12 str. have no hpaB or hpaC gene.

Source

This part was cloned from E. coli BL21(DE3) genome by PCR. We added restriction sites on both ends of the genomic sequence.

Links to NCBI gene detabase

http://www.ncbi.nlm.nih.gov/gene/8183130 (hpaB)

http://www.ncbi.nlm.nih.gov/gene/8183129 (hpaC)

References