Difference between revisions of "Part:BBa K1031015"
 
Line 10: Line 10:
 
== '''Components and Characterization''' ==
 
== '''Components and Characterization''' ==
  
−
K1031015 consists of <html><a href="https://parts.igem.org/Part:BBa_J61051">NahR</a></html>, Psal promoter, RBS <html><a href="https://parts.igem.org/Part:BBa_B0032">B0032</a></html> and transcription factor CƖ. CƖ functions as a repressor of hybrid promoter[https://parts.igem.org/Part:BBa_K1031021]. It is the repression branch of the Band-pass Filter circuit ('''Fig 1'''). In order to fine-tune the expression level of transcriptional repressor, we adjusted the RBS (Ribosome Binding Site) intensity in front of CƖ. K1031015 adopts RBS B0032.   
+
K1031015 consists of <html><a href="https://parts.igem.org/Part:BBa_J61051">NahR</a></html>, Psal promoter, RBS <html><a href="https://parts.igem.org/Part:BBa_B0032">B0032</a></html> and transcription factor CƖ. CƖ functions as a repressor of <html><a href="https://parts.igem.org/Part:BBa_K1031021">hybrid promoter</a></html>. It is the repression branch of the Band-pass Filter circuit ('''Fig 1'''). In order to fine-tune the expression level of transcriptional repressor, we adjusted the RBS (Ribosome Binding Site) intensity in front of CƖ. K1031015 adopts RBS B0032.   
  
 
<html>
 
<html>

Latest revision as of 21:15, 27 September 2013

NahR-Psal-B0032-RiboJ-CI

For detailed information concerning Band-pass Filter, please visit 2013 Peking iGEM Band-pass Filter


Components and Characterization

K1031015 consists of NahR, Psal promoter, RBS B0032 and transcription factor CƖ. CƖ functions as a repressor of hybrid promoter. It is the repression branch of the Band-pass Filter circuit (Fig 1). In order to fine-tune the expression level of transcriptional repressor, we adjusted the RBS (Ribosome Binding Site) intensity in front of CƖ. K1031015 adopts RBS B0032.

Fig 1 Repression circuit of Band-pass Filter. The orange square represents transcription factor NahR activating Psal promoter shown in light blue. Once Psal promoter is initiated, CƖ repressor, which is shown as square in dark blue, is expressed, inhibiting hybrid promoter marked with two colors, thus inhibiting expression of sfGFP. The RBS B0032 is shown in light green, regulating the expression level of CƖ protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 408
  • 1000
    COMPATIBLE WITH RFC[1000]