Difference between revisions of "Part:BBa J31000:Design"
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===Biobricks=== | ===Biobricks=== | ||
| − | The Biobricks attached to this part are not | + | The Biobricks attached to this part are not wild type but the cut sites are still viable. |
| − | <font color='blue'>GAATTC</font color><font color='brown'>GCGGCCGC</font color><font color='green'>T</font color><font color='orange'>TCTAGA</font color><font color='red'>-</font color><u> | + | <font face=courier new><font color='blue'>GAATTC</font color><font color='brown'>GCGGCCGC</font color><font color='green'>T</font color><font color='orange'>TCTAGA</font color><font color='red'>-</font color><u>ATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTACCAGTGCAAATTGTGACCGCATTTTTGAGGACCGTATCAGTGGCAAGATTGCAAACCGCCCCGGCCTGAAACGAGCGTTAA |
| + | |||
| + | AGTATGTAAATAAAGGCGATACTCTTGTCGTCTGGAAATTAGACAGACTGGGCCGCAGCGTGAAAAACCTGGTGGCGTTAATATCAGAATTACATGAACGTGGAGCTCACTTCCATTCTTTAACCGATAGTATTGATACCAGTAGCGCGATGGGGCGAT | ||
| + | |||
| + | TCTTTTTTCATGTAATGTCAGCACTGGCCGAGATGGAGCGAGAATTAATTGTCGAGCGAACCCTTGCCGGACTGGCTGCCGCCAGAGCGCAAGGACGACTGGGAGGGCGCCCTCGGGCGATCAACAAACATGAACAGGAACAGATTAGTCGGCTATTA | ||
| + | |||
| + | GAGAAAGGCCATCCTCGGCAGCAACTAGCTATTATTTTTGGTATTGGCGTATCTACCTTATACAGATATTTTCCGGCAAGCCGTATAAAAAAACGAATGAATTAA</u><font color='tan'>-</font color><font color='lime green'>ACTAGT | ||
| + | </font color><font color='navy'>-</font color><font color='pink'>GCGGCCGC</font color><font color='cyan'>CTGCAG</font color></font face> | ||
There is no <font color='red'>G</font color> spacer between the <font color='orange'>XbaI</font color> and the <u>insert</u>. | There is no <font color='red'>G</font color> spacer between the <font color='orange'>XbaI</font color> and the <u>insert</u>. | ||
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[[Image:BioBricks_from_paper.png]] | [[Image:BioBricks_from_paper.png]] | ||
| − | |||
===Source=== | ===Source=== | ||
Latest revision as of 14:26, 27 July 2006
DNA-invertase Hin from Salmonella typhimurium
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is cloned into plasmid pSB1A2.
Biobricks
The Biobricks attached to this part are not wild type but the cut sites are still viable.
GAATTCGCGGCCGCTTCTAGA-ATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTACCAGTGCAAATTGTGACCGCATTTTTGAGGACCGTATCAGTGGCAAGATTGCAAACCGCCCCGGCCTGAAACGAGCGTTAA
AGTATGTAAATAAAGGCGATACTCTTGTCGTCTGGAAATTAGACAGACTGGGCCGCAGCGTGAAAAACCTGGTGGCGTTAATATCAGAATTACATGAACGTGGAGCTCACTTCCATTCTTTAACCGATAGTATTGATACCAGTAGCGCGATGGGGCGAT
TCTTTTTTCATGTAATGTCAGCACTGGCCGAGATGGAGCGAGAATTAATTGTCGAGCGAACCCTTGCCGGACTGGCTGCCGCCAGAGCGCAAGGACGACTGGGAGGGCGCCCTCGGGCGATCAACAAACATGAACAGGAACAGATTAGTCGGCTATTA
GAGAAAGGCCATCCTCGGCAGCAACTAGCTATTATTTTTGGTATTGGCGTATCTACCTTATACAGATATTTTCCGGCAAGCCGTATAAAAAAACGAATGAATTAA-ACTAGT -GCGGCCGCCTGCAG
There is no G spacer between the XbaI and the insert.
There is no T spacer between the insert and the SpeI site.
There is no A spacer between the SpeI site and the NotI site.
The last C of the NotI site is not conserved with the initial C from the PstI site.
The BB suffix currently has this sequence for NotI and PstI: GCGGCCGcCTGCAG
But it should have been: GCGGCCGCTGCAG
There is no spacer between the NotI site and the SpeI site. There should be a T.
We compared our BioBricks with those from Tom Knight's paper, Idempotent Vector Design for Standard Assembly of Biobricks. As seen below
Source
Salmonella typhimurium
References
Knight, Tom. Idempotent Vector Design for Standard Assembly of Biobricks