Difference between revisions of "Part:BBa K1020008"
 
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<partinfo>BBa_K1020008 short</partinfo>
 
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This part is composed of genes encoding two enzymes,NPDC and AAR. Both are key enzymes in the production of fungible hydrocarbon biofuels, for they play important roles in turning fatty acyl-ACPs into alkanes.Different from BBa_K590025 given by Washington iGEM 2010, we have done the coden optimization for ''E.coli''.And the sequence is completely different.
 
 
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<p>This part is composed of genes encoding two enzymes,NPDC and AAR. Both are key enzymes in the production of fungible hydrocarbon biofuels, for they play important roles in turning fatty acyl-ACPs into alkanes.Different from BBa_K590025 given by Washington iGEM 2010, we have done the coden optimization for ''E.coli''.And the sequence is completely different.</p>
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<img src="https://static.igem.org/mediawiki/igem.org/4/44/PR_K1020008_1.jpg"/>
 
<img src="https://static.igem.org/mediawiki/igem.org/4/44/PR_K1020008_1.jpg"/>
 
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We constructed 3 strains to characterize the productivity of intracellular alkane:
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strain1:No.53：producing module (constitutive promoter J23100)+ Alk-Sensor device
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strain2:No.54：producing module (constitutive promoter J23114) + Alk-Sensor device
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strain3:No.55：Null+ Alk-Sensor device
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The promoter strength of No.54 is stronger than that of No.53. Theorectically,  alkane productivity of No.53 and No.54 will be different, and intracellular alkane concentration of the above 3 strains will be different-- No.54 the highest, No.53 lower and No.55 the lowest.
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<img src="https://static.igem.org/mediawiki/igem.org/a/ad/PR_K1020008_2.jpg" width="700px"/>
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The 3 strains are cultured in 3ml M9 medium for about 24 hours.And the vertical coordinate of picture below is fluorescent indensity per OD.
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<img src="https://static.igem.org/mediawiki/igem.org/5/53/PR_K1020008_3.jpg"/>
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The fluorescent indensity of No.54 is the stronger than No.53, which indirectly proves that this module produce alkanes.
  
 
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Latest revision as of 20:28, 27 September 2013

alkane producing module

This part is composed of genes encoding two enzymes,NPDC and AAR. Both are key enzymes in the production of fungible hydrocarbon biofuels, for they play important roles in turning fatty acyl-ACPs into alkanes.Different from BBa_K590025 given by Washington iGEM 2010, we have done the coden optimization for ''E.coli''.And the sequence is completely different.

We constructed 3 strains to characterize the productivity of intracellular alkane:

strain1:No.53：producing module (constitutive promoter J23100)+ Alk-Sensor device

strain2:No.54：producing module (constitutive promoter J23114) + Alk-Sensor device

strain3:No.55：Null+ Alk-Sensor device

The promoter strength of No.54 is stronger than that of No.53. Theorectically, alkane productivity of No.53 and No.54 will be different, and intracellular alkane concentration of the above 3 strains will be different-- No.54 the highest, No.53 lower and No.55 the lowest.

The 3 strains are cultured in 3ml M9 medium for about 24 hours.And the vertical coordinate of picture below is fluorescent indensity per OD.

The fluorescent indensity of No.54 is the stronger than No.53, which indirectly proves that this module produce alkanes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 48
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]