Difference between revisions of "Part:BBa K1124009"
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===Usage and Biology=== | ===Usage and Biology=== | ||
'''The Effects on tyrosine metabolism of TyrR''' | '''The Effects on tyrosine metabolism of TyrR''' | ||
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TyrR is a transcriptional regulator which represses the expression of several enzymes involved in the tyrosine producing pathway in ''E. coli''. | TyrR is a transcriptional regulator which represses the expression of several enzymes involved in the tyrosine producing pathway in ''E. coli''. | ||
It was shown that TyrR repression leaded to increase of the tyrosine production. | It was shown that TyrR repression leaded to increase of the tyrosine production. | ||
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'''RNA Silencing Utilizing MicC sRNA Scaffold''' | '''RNA Silencing Utilizing MicC sRNA Scaffold''' | ||
| − | The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold([[BBa_K1124005]]) works as a tool of RNA silencing (see descriptions in MicC sRNA scaffold([[BBa_K1124005]])). In this part,anti-tyrR sRNA, the target-binding sequence is complementary to the first 24bp of tyrR CDS(that is, the sequence from start codon ATG and the following 7 triplet codons). | + | The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold([[Part:BBa_K1124005]]) works as a tool of RNA silencing (see descriptions in MicC sRNA scaffold([[Part:BBa_K1124005]])). In this part,anti-tyrR sRNA, the target-binding sequence is complementary to the first 24bp of tyrR CDS(that is, the sequence from start codon ATG and the following 7 triplet codons). |
We expect that this part will repress ''tyrR'' expression in post-transcriptional way and therefore will increase tyrosine production. | We expect that this part will repress ''tyrR'' expression in post-transcriptional way and therefore will increase tyrosine production. | ||
| − | With this part, we constructed tyrosine synthesis device([[BBa_K1124106]]), which can likely increase tyrosine production. | + | With this part, we constructed tyrosine synthesis device([[Part:BBa_K1124106]]), which can likely increase tyrosine production. |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 20:13, 27 September 2013
anti-tyrR sRNA
Usage and Biology
The Effects on tyrosine metabolism of TyrR
TyrR is a transcriptional regulator which represses the expression of several enzymes involved in the tyrosine producing pathway in E. coli. It was shown that TyrR repression leaded to increase of the tyrosine production.
RNA Silencing Utilizing MicC sRNA Scaffold
The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold(Part:BBa_K1124005) works as a tool of RNA silencing (see descriptions in MicC sRNA scaffold(Part:BBa_K1124005)). In this part,anti-tyrR sRNA, the target-binding sequence is complementary to the first 24bp of tyrR CDS(that is, the sequence from start codon ATG and the following 7 triplet codons). We expect that this part will repress tyrR expression in post-transcriptional way and therefore will increase tyrosine production. With this part, we constructed tyrosine synthesis device(Part:BBa_K1124106), which can likely increase tyrosine production.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]