Difference between revisions of "Part:BBa K1100009"
 
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<partinfo>BBa_K1100009 short</partinfo>
 
<partinfo>BBa_K1100009 short</partinfo>
  
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This part is a intermediate.A seious of parts are put together onto the pSB1C3 plasmid including the promter(BBa_R0040),csy4 insulator(16-nt), a RBS(BBa_B0034) and the Csy4 gene.Therefore, it can be add upstream of any terminator you are interested in. With a sfGFP reporter with a insulator -- the 16nt Csy4 cleavage loci, you can estimate how downstream sequence influences the terminator efficiency, like the study we did with the part BBa_K1100011.
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This part is a intermediate.A seious of parts are put together onto the pSB1C3 plasmid including the promter(BBa_R0040),csy4 insulator(16-nt), a RBS(BBa_B0034) and the Csy4 gene.Therefore, it can be add upstream of any terminator you are interested in. With a sfGFP reporter with a insulator -- the 16nt Csy4 cleavage loci, you can estimate how downstream sequence influences the terminator efficiency, like the study we did with the part [https://parts.igem.org/Part:BBa_K1100011 BBa_K1100011].
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1100010 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1100009 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1100010 parameters</partinfo>
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<partinfo>BBa_K1100009 parameters</partinfo>
 
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Latest revision as of 22:04, 27 September 2013

Csy4 generator with no terminator

This part is a intermediate.A seious of parts are put together onto the pSB1C3 plasmid including the promter(BBa_R0040),csy4 insulator(16-nt), a RBS(BBa_B0034) and the Csy4 gene.Therefore, it can be add upstream of any terminator you are interested in. With a sfGFP reporter with a insulator -- the 16nt Csy4 cleavage loci, you can estimate how downstream sequence influences the terminator efficiency, like the study we did with the part BBa_K1100011.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 164