Difference between revisions of "Part:BBa K1051801"
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<p>This part consists of promoter SNR52, sgRNA coding sequence and SUP4 terminator. | <p>This part consists of promoter SNR52, sgRNA coding sequence and SUP4 terminator. | ||
| − | Structure of sgRNA is like the picture below</p> | + | <br/>Structure of sgRNA is like the picture below</p> |
https://static.igem.org/mediawiki/parts/5/56/Structure_of_sgRNA.png | https://static.igem.org/mediawiki/parts/5/56/Structure_of_sgRNA.png | ||
| − | <br/>sgRNA can help dCas9(BBa_k1051800) to target to <i>hubi</i> to inhibit the expression of <i>hubi</i>. | + | <br/>This sgRNA can help dCas9(BBa_k1051800) to target to <i>hubi</i> to inhibit the expression of <i>hubi</i>. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 18:57, 27 September 2013
Targeted to HUBI gene ATG downstream position of the 12 bp sgRNA, cooperate to dCas 9 protein, inhib
This part consists of promoter SNR52, sgRNA coding sequence and SUP4 terminator.
Structure of sgRNA is like the picture below
This sgRNA can help dCas9(BBa_k1051800) to target to hubi to inhibit the expression of hubi.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 134
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]